| Detailed Information for Phage MementoMori |
| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NWU1 |
| Found By | Elijah Washburn |
| Year Found | 2016 |
| Location Found | LaVista, NE USA |
| Finding Institution | Nebraska Wesleyan University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 67°C |
| GPS Coordinates | 41.187567 N, 95.972347 W Map |
| Discovery Notes | This phage was isolated near the base of an oak tree. I dug about five inches down attempting to find soil near a root. |
| Naming Notes | This phage had very small plaques from the very first isolation. The plaques size and number made it difficult to tell if I had truly isolated it, as well it was very difficult to find the correct titre to get a webbed plate, I persevered through the challenges and eventually completed my work on the phage. I decided to name it memento mori in honor of the great tradition the ancient Romans held after victory, memento mori is meant to remind me to be humble while i think of my work here, and to thank all those who helped me through it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1128 |
| Genome length (bp) | 55572 |
| Character of genome ends | Circularly Permuted |
| GC Content | 70.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EI |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Nebraska Wesleyan University |
| Annotation Status | In GenBank |
| Plaque Notes | This phage had very small clear plaques, that were circular and often seemed to overlap each other at lower titres. |
| Number of Genes | 96 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH271303 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
