| Detailed Information for Phage Mendokysei |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brandon Yee |
| Year Found | 2008 |
| Location Found | Santa Cruz, CA USA |
| Finding Institution | University of California, Santa Cruz |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.9988 N, 122.052483 W Map |
| Discovery Notes | Taken from a soil sample at depth <1inch, found inside the Merril Gardens Irrigation Box underneath the irrigation piping. The irrigation box is at the top of the hill near the entrance, and is for the third row of crops from the entrance. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 14, 2012 |
| Genome length (bp) | 43511 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGAACGCCTT |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | T |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of California, Santa Cruz |
| Annotation Status | In GenBank |
| Plaque Notes | 0.5mm clear plaques.
24-48 hour incubation period in 37°C conditions
|
| Morphotype | Siphoviridae |
| Number of Genes | 65 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG925349 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2008UCSCMendokyseiBY |
| SEA Lysate Titer |
4.0x10^11 |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
D4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
