| Detailed Information for Phage MillySue | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Camille Bracy |
| Year Found | 2025 |
| Location Found | Durham, NC USA |
| Finding Institution | North Carolina Central University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 77°C |
| GPS Coordinates | 35.97501 N, 78.90234 W Map |
| Discovery Notes | Soil was collected in a shaded area of a garden bed with visible plants growing. Soil was damp and moist, and a rich dark brown color. |
| Naming Notes | MillySue is my dad's nickname for me, so I wanted to name my phage after something meaningful to me. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 209 |
| Genome length (bp) | 42145 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | TCCGCCGCGTAA |
| GC Content | 65.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FF |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | North Carolina Central University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Clear plaques with some slight variation in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMillySue_1 MillySue_2 MillySue_3 — tRNA MillySue_4 MillySue_5 MillySue_6 MillySue_7 MillySue_8 MillySue_9 MillySue_10 MillySue_11 MillySue_12 MillySue_13 MillySue_14 MillySue_15 MillySue_16 MillySue_17 MillySue_18 MillySue_19 MillySue_20 MillySue_21 MillySue_22 MillySue_23 MillySue_24 MillySue_25 MillySue_26 MillySue_27 MillySue_28 MillySue_29 MillySue_30 MillySue_31 MillySue_32 MillySue_33 MillySue_34 MillySue_35 MillySue_36 MillySue_37 MillySue_38 — tRNA MillySue_39 MillySue_40 MillySue_41 MillySue_42 MillySue_43 MillySue_44 MillySue_45 MillySue_46 MillySue_47 MillySue_48 MillySue_49 MillySue_50 MillySue_51 — tRNA MillySue_52 MillySue_53 MillySue_54 MillySue_55 MillySue_56 MillySue_57 MillySue_58 MillySue_59 MillySue_60 MillySue_61 MillySue_62 MillySue_63 MillySue_64 MillySue_65 MillySue_66 MillySue_67 MillySue_68 MillySue_69 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 9.2x10^11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |