Gordonia phage Neville
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Detailed Information for Phage Neville
Discovery Information
Isolation HostGordonia terrae 3612
Found ByLauren Andrews and Emma Bragdon
Year Found2017
Location FoundWestford, MA USA
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates42.582525 N, 71.485782 W Map
Discovery NotesSoil sample taken from a garden/pumpkin patch.
Naming NotesWe both like Harry Potter, and Neville Longbottom loves plants. This phage was discovered in a pumpkin patch so we thought why not name it Neville.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 6, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage405
Genome length (bp)75813
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceATCTGCCCCAC
GC Content58.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDU
SubclusterDU2
Cluster Life CycleUnknown
Lysogeny NotesNeville is a temperate phage and forms lysogens.
Other Cluster Members
Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
Plaque NotesThe plaques are turbid with a diameter of 2-3mm.
Number of Genes129
Number of tRNAs7
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Neville_1

Neville_2

Neville_3

Neville_4

Neville_5

Neville_6

Neville_7

Neville_8
(tyrosine integrase)

Neville_9

Neville_10

Neville_11
(terminase small subunit)

Neville_12
(terminase large subunit)

Neville_13
(portal protein)

Neville_14
(MuF-like minor capsid protein)

Neville_15
(scaffolding protein)

Neville_16
(major capsid protein)

Neville_17
(head-to-tail adaptor)

Neville_18
(head-to-tail stopper)

Neville_19

Neville_20
(tail terminator)

Neville_21
(major tail protein)

Neville_22

Neville_23
(tail assembly chaperone)

Neville_24
(tail assembly chaperone)

Neville_25

Neville_26
(tape measure protein)

Neville_27
(minor tail protein)

Neville_28
(minor tail protein)

Neville_29
(minor tail protein)

Neville_30
(minor tail protein)

Neville_31

Neville_32
(minor tail protein)

Neville_33

Neville_34
(lysin A, protease C39 domain)

Neville_35
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Neville_36

Neville_37

Neville_38
(Lsr2-like DNA bridging protein)

Neville_39

Neville_40
(immunity repressor)

Neville_41
(Cro protein)

Neville_42
(excise)

Neville_43

Neville_44

Neville_45

Neville_46

Neville_47

Neville_48
(phosphoribosyltransferase)

Neville_49

Neville_50

Neville_51

Neville_52

Neville_53

Neville_54

Neville_55
(MazG-like nucleotide pyrophosphohydrolase)

Neville_56

Neville_57

Neville_58
(Cas4 family exonuclease)

Neville_59

Neville_60
(ERF family ssDNA binding protein)

Neville_61

Neville_62
(WhiB family transcription factor)

Neville_63
(WhiB family transcription factor)

Neville_64

Neville_65

Neville_66
(DNA methylase)

Neville_67

Neville_68

Neville_69

Neville_70

Neville_71
(DnaB-like dsDNA helicase)

Neville_72
(ssDNA binding protein)

Neville_73

Neville_74

Neville_75
(DNA methylase)

Neville_76
(RusA-like resolvase)

Neville_77

Neville_78
(polynucleotide kinase)

Neville_79

Neville_80
(RNA ligase)

Neville_81
(phosphoribosyl pyrophosphate transferase)

Neville_82

Neville_83

Neville_84

Neville_85
(nucleotidyl transferase)

Neville_86

Neville_87

Neville_88

Neville_89

Neville_90

Neville_91
(Ro-like RNA binding protein)

Neville_92

Neville_93
(DNA primase/polymerase)

Neville_94 — tRNA
(tRNA-His(gtg))

Neville_95 — tRNA
(tRNA-Tyr(gta))

Neville_96 — tRNA
(tRNA-Gly(tcc))

Neville_97 — tRNA
(tRNA-Lys(ctt))

Neville_98 — tRNA
(tRNA-Leu(caa))

Neville_99

Neville_100

Neville_101

Neville_102

Neville_103 — tRNA
(tRNA-Gln(ctg))

Neville_104 — tRNA
(tRNA-Tyr(gta))

Neville_105

Neville_106
(ThyX-like thymidylate synthase)

Neville_107

Neville_108

Neville_109

Neville_110
(ribonucleotide reductase)

Neville_111
(NrdH-like glutaredoxin)

Neville_112

Neville_113
(MRE11 double-strand break endo/exonuclease)

Neville_114

Neville_115
(lysin B)

Neville_116

Neville_117

Neville_118
(AAA-ATPase)

Neville_119
(NHN endonuclease)

Neville_120
(glycosyltransferase)

Neville_121

Neville_122
(glycosyltransferase)

Neville_123

Neville_124
(glycosyltransferase)

Neville_125
(HNH endonuclease)

Neville_126

Neville_127

Neville_128

Neville_129

Neville_130

Neville_131

Neville_132
(helix-turn-helix DNA binding domain protein)

Neville_133

Neville_134

Neville_135

Neville_136

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH651182
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 73
Pitt Freezer Box Grid# B6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload