Mycobacterium phage Ntise
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| Detailed Information for Phage Ntise | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Molopo Johannes Lipali |
| Year Found | 2024 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.957005 S, 18.466879 E Map |
| Discovery Notes | The soil, collected approximately 10 cm deep, came from a fertilized flower garden at the University of Cape Town's middle campus right after the garden was watered. The soil was enriched with M. smegmatis for 24 hours to acquire plaques, and plaque plates were prepared via spotting and incubated for 48 hours. |
| Naming Notes | The phage is named after my late sister. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The initial plaques acquired from enriched soil filtrates, prior to the purification of phages, were round and clear in the middle with a turbid halo surrounding the clear zone. After purification, there was a combination of turbid and clear round plaques on the dilution plates prepared. A 10^-4 plate with both turbid and clear plaques was used to sample the phages for sequencing. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
