| Detailed Information for Phage Oaker | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle DeNigris |
| Year Found | 2012 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.971361 N, 75.194733 W Map |
| Discovery Notes | Soil taken from gazelle exhibit at the Philadelphia Zoo. Soil was in a shady area near kopje and was 2-2.5 cm deep. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 19, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 250 |
| Genome length (bp) | 69099 |
| Character of genome ends | Circularly Permuted |
| GC Content | 57.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | H |
| Subcluster | H1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were fast growing appearing after about 28 hours but not as soon as 24 hours. The plaques were small (about 1 mm in diameter) and very clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewOaker_1 Oaker_2 Oaker_3 Oaker_4 Oaker_5 Oaker_6 Oaker_7 Oaker_8 Oaker_9 Oaker_10 Oaker_11 Oaker_12 Oaker_13 Oaker_14 Oaker_15 Oaker_16 Oaker_17 Oaker_18 Oaker_19 Oaker_20 Oaker_21 Oaker_22 Oaker_23 Oaker_24 Oaker_25 Oaker_26 Oaker_27 Oaker_28 Oaker_29 Oaker_30 Oaker_31 Oaker_32 Oaker_33 Oaker_34 Oaker_35 Oaker_36 Oaker_37 Oaker_38 Oaker_39 Oaker_40 Oaker_41 Oaker_42 Oaker_43 Oaker_44 Oaker_45 Oaker_46 Oaker_47 Oaker_48 Oaker_49 Oaker_50 Oaker_51 Oaker_52 Oaker_53 Oaker_54 Oaker_55 Oaker_56 Oaker_57 Oaker_58 Oaker_59 Oaker_60 Oaker_61 Oaker_62 Oaker_63 Oaker_64 Oaker_65 Oaker_66 Oaker_67 Oaker_68 Oaker_69 Oaker_70 Oaker_71 Oaker_72 Oaker_73 Oaker_74 Oaker_75 Oaker_76 Oaker_77 Oaker_78 Oaker_79 Oaker_80 Oaker_81 Oaker_82 Oaker_83 Oaker_84 Oaker_85 Oaker_86 Oaker_87 Oaker_88 Oaker_89 Oaker_90 Oaker_91 Oaker_92 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF986247 |
| Refseq Number | NC_023578 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2012STJOoakerDMD |
| SEA Lysate Titer | 6.1 x 10^10 pfu/mL |
| Date of SEA Lysate Titering | Oct 18, 2012 |
| Pitt Freezer Box# | 39 |
| Pitt Freezer Box Grid# | 37 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
