| Detailed Information for Phage Oaker |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle DeNigris |
| Year Found | 2012 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.971361 N, 75.194733 W Map |
| Discovery Notes | Soil taken from gazelle exhibit at the Philadelphia Zoo. Soil was in a shady area near kopje and was 2-2.5 cm deep. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 19, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 250 |
| Genome length (bp) | 69099 |
| Character of genome ends | Circularly Permuted |
| GC Content | 57.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | H |
| Subcluster | H1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were fast growing appearing after about 28 hours but not as soon as 24 hours. The plaques were small (about 1 mm in diameter) and very clear. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF986247 |
| Refseq Number | NC_023578 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012STJOoakerDMD |
| SEA Lysate Titer |
6.1 x 10^10 pfu/mL |
| Date of SEA Lysate Titering |
Oct 18, 2012 |
| Pitt Freezer Box# |
39 |
| Pitt Freezer Box Grid# |
37 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
