Detailed Information for Phage Phreeze |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Rina Watanabe |
Year Found | 2017 |
Location Found | Los Angeles, CA |
Finding Institution | University of California, Los Angeles |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | 30°C |
GPS Coordinates | 34.04604 N, 118.29136 W Map |
Discovery Notes | Phage Phreeze was isolated from moist soil collected on 10/08/17 in Korea Town (Los Angeles) by Rina Watanabe. Air temperature at time of collection was 20°C. Phreeze was isolated by direct plating at 30°C.
Characterization was performed by Keith Mitchell.
Midi-Lysate (Archive) Titer: 8.25*10^12 PFU/mL Date of High-Titer Lysate Titration: 11/29/17
MIMG103AL-F17 Instructor: Dr. Amanda Freise Teaching Assistant: Andrew Lund Undergraduate Assistant: Keith Mitchell Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker |
Naming Notes | Named after course instructor Dr. Amanda Freise. Both the name of the instructor and the name of the phage are pronounced "freeze." |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 2, 2018 |
Genome length (bp) | 67906 |
Character of genome ends | Unknown |
GC Content | 57.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | H |
Subcluster | H1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | University of California, Los Angeles |
Annotation Status | In GenBank |
Plaque Notes | small turbid plaques, 0.5mm |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MN010756 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
58 |
Pitt Freezer Box Grid# |
F6 |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |