| Detailed Information for Phage Opie | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Former names | Poesie |
| Found By | Emma Gay |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.443298 N, 79.955935 W Map |
| Discovery Notes | This phage was isolated from a sample obtained from a flower bed outside Holland Hall at the University of Pittsburgh. The soil sample was wet due to overnight rains. Vegetation present included some flowers and small weeds. Insect life was also present in the soil. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1231 |
| Genome length (bp) | 47713 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGGTA |
| GC Content | 67.0% |
| Sequencing Notes | at position 32434 bp approximately 49% of reads contain a string of 13-14 G's, consensus 12 G's |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DB |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques formed are halo in shape. The center of the halos are medium in size and clear. The halo that surround the clearing is cloudy. |
| Morphotype | Siphoviridae |
| Number of Genes | 73 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewOpie_1 Opie_2 Opie_3 Opie_4 Opie_5 Opie_6 Opie_7 Opie_8 Opie_9 Opie_10 Opie_11 Opie_12 Opie_13 Opie_14 Opie_15 Opie_16 Opie_17 Opie_18 Opie_19 Opie_20 Opie_21 Opie_22 Opie_23 Opie_24 Opie_25 Opie_26 Opie_27 Opie_28 Opie_29 Opie_30 Opie_31 Opie_32 Opie_33 Opie_34 Opie_35 Opie_36 Opie_37 Opie_38 Opie_39 Opie_40 Opie_41 Opie_42 Opie_43 Opie_44 Opie_45 Opie_46 Opie_47 Opie_48 Opie_49 Opie_50 Opie_51 Opie_52 Opie_53 Opie_54 Opie_55 Opie_56 Opie_57 Opie_58 Opie_59 Opie_60 Opie_61 Opie_62 Opie_63 Opie_64 Opie_65 Opie_66 Opie_67 Opie_68 Opie_69 Opie_70 Opie_71 Opie_72 Opie_73 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN813694 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 57 |
| Pitt Freezer Box Grid# | H4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
