Arthrobacter phage PeggyLeg03
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| Detailed Information for Phage PeggyLeg03 | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Baylee Jauquet |
| Year Found | 2021 |
| Location Found | Viroqua, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.494722 N, 90.863889 W Map |
| Discovery Notes | A mix of wet and dry soil, near dog's potty spot, no roots, damp from light rain early in the day, no grass near the soil sample Soil sample by Tahmara Henderickson |
| Naming Notes | Named after a 3 legged squirrel named Peggy that was rehabbed from a baby to an adult. I chose the name because Peggy was my favorite squirrel to rehab. She was playful, a sweetheart, and a cuddler |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 23, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 606 |
| Genome length (bp) | 43268 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGAGA |
| GC Content | 65.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques stayed around the 1mm size, around the average size from others in the class, with being turbid at the beginning of the process, until they got more clearer around Plaque purification rounds 1 and 2. With some of the plaques having faint haloes. |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ995425 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 140 |
| Pitt Freezer Box Grid# | D1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
