| Detailed Information for Phage PeggyLeg03 | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Baylee Jauquet |
| Year Found | 2021 |
| Location Found | Viroqua, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.494722 N, 90.863889 W Map |
| Discovery Notes | A mix of wet and dry soil, near dog's potty spot, no roots, damp from light rain early in the day, no grass near the soil sample Soil sample by Tahmara Henderickson |
| Naming Notes | Named after a 3 legged squirrel named Peggy that was rehabbed from a baby to an adult. I chose the name because Peggy was my favorite squirrel to rehab. She was playful, a sweetheart, and a cuddler |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 23, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 606 |
| Genome length (bp) | 43268 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGAGA |
| GC Content | 65.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques stayed around the 1mm size, around the average size from others in the class, with being turbid at the beginning of the process, until they got more clearer around Plaque purification rounds 1 and 2. With some of the plaques having faint haloes. |
| Morphotype | Siphoviridae |
| Number of Genes | 70 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPeggyLeg03_1 PeggyLeg03_2 PeggyLeg03_3 PeggyLeg03_4 PeggyLeg03_5 PeggyLeg03_6 PeggyLeg03_7 PeggyLeg03_8 PeggyLeg03_9 PeggyLeg03_10 PeggyLeg03_11 PeggyLeg03_12 PeggyLeg03_13 PeggyLeg03_14 PeggyLeg03_15 PeggyLeg03_16 PeggyLeg03_17 PeggyLeg03_18 PeggyLeg03_19 PeggyLeg03_20 PeggyLeg03_21 PeggyLeg03_22 PeggyLeg03_23 PeggyLeg03_24 PeggyLeg03_25 PeggyLeg03_26 PeggyLeg03_27 PeggyLeg03_28 PeggyLeg03_29 PeggyLeg03_30 PeggyLeg03_31 PeggyLeg03_32 PeggyLeg03_33 PeggyLeg03_34 PeggyLeg03_35 PeggyLeg03_36 PeggyLeg03_37 PeggyLeg03_38 PeggyLeg03_39 PeggyLeg03_40 PeggyLeg03_41 PeggyLeg03_42 PeggyLeg03_43 PeggyLeg03_44 PeggyLeg03_45 PeggyLeg03_46 PeggyLeg03_47 PeggyLeg03_48 PeggyLeg03_49 PeggyLeg03_50 PeggyLeg03_51 PeggyLeg03_52 PeggyLeg03_53 PeggyLeg03_54 PeggyLeg03_55 PeggyLeg03_56 PeggyLeg03_57 PeggyLeg03_58 PeggyLeg03_59 PeggyLeg03_60 PeggyLeg03_61 PeggyLeg03_62 PeggyLeg03_63 PeggyLeg03_64 PeggyLeg03_65 PeggyLeg03_66 PeggyLeg03_67 PeggyLeg03_68 PeggyLeg03_69 PeggyLeg03_70 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ995425 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 140 |
| Pitt Freezer Box Grid# | D1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
