Arthrobacter phage PorkBelly
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| Detailed Information for Phage PorkBelly | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Jose Zamora Lopez, Teams New Genes, Paciphic Rim |
| Year Found | 2025 |
| Location Found | Pasadena, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | Taken from outside of a garden. I took it in a shaded area. Depth from which sample was taken (in cm): 3 cm Air temperature if sample collected outdoors (in degrees Celsius): 17.8 C (64 F) Host-Enrichment using Arthrobacter sulfureus (A. sulf), Arthrobacter species (A. sp), Arthrobacter atrocyaneus (A. atro), and Arthrobacter globiformis (A. globi). The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: Instructor: Dr. Tejas Bouklas Teaching Assistant: Pranava Jana Undergraduate Assistant(s): Sarah, Ryan Team #1 Name: Paciphic Rim Team #1 Members: Nolan Reber, Pranay Sashikanth, Hayden Kim, Arman Bhatnagar Team #2 Name: NewGenes Team #2 Members: Marcus Lee, Megan Thanislas, Nick Wilson, Chrissy Tetreault |
| Naming Notes | Marcus’s favorite Korean food |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Between plaque assays, morphology varied from pinpoint to clear to halo Description of plaque morphology: Clear, slightly turbid (varies) Average size (and size range) diameter in mm: 2.5-3 (decreased in further weeks, likely decreased titer) |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
