Mycobacterium phage Rigel
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Detailed Information for Phage Rigel
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEric Valery
Year Found2012
Location FoundWilliamsburg, VA USA
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.268889 N, 76.712222 W Map
Discovery NotesSoil sample from 5" deep
Damp
Warm weather
Shaded environment
Naming NotesI named it after the six brightest star in the sky.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterA
SubclusterA1
Cluster Life CycleTemperate
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesMy plaques, coming out of a 24 hour incubation looked nice. There were no abnormal or unshapely plaques. Plates were free from turbidity. However, there was a situation when an incubation occurred for 7 days unintentionally. It was observed that my plaque's size was abnormally larger in comparison to my previous plaques. A hypothesis was made saying that my phage, when it undergoes a longer incubation period, continues to lyse bacteria causing the plaques to grow.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer Greater than 10^-6 PFUs per ml
Date of SEA Lysate Titering Dec 7, 2012
Pitt Freezer Box# 35
Pitt Freezer Box Grid# 10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload