Detailed Information for Phage Strobilo |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Kate McAvaddy and Rachel Vemula |
Year Found | 2023 |
Location Found | Mays Landing, NJ United States of America |
Finding Institution | Saint Joseph's University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 39.46376 N, 74.74224 W Map |
Discovery Notes | The soil was collected near a trail at Lake Lenape in Mays Landing, New Jersey. I dug around 40.6 centimeters into the ground in between a tree and a mushroom and collected the sample which was slightly sandy and wet. |
Naming Notes | The soil was obtained from a spot located next to a fungus known as "Old Man of the Woods." The scientific name for this fungus is strobilomyces strobilaceus, so I named the phage "strobilo" for short. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 12, 2024 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 3287 |
Genome length (bp) | 58127 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CTCGCGGCAT |
GC Content | 68.3% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | K |
Subcluster | K2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Saint Joseph's University |
Annotation Status | In GenBank |
Plaque Notes | The plaques were turbid and typically around 2 mm. Plaques typically emerged after 48 hours. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | PP978785 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
187 |
Pitt Freezer Box Grid# |
A2 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |