The Actinobacteriophage Database

Mycobacterium phage Send513

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Detailed Information for Phage Send513
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Amanda Hoppers
Year Found	2009
Location Found	Foster Falls, TN USA
Finding Institution	Georgia State University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	35.182028 N, 85.673895 W Map
Discovery Notes	small eddy in a pool under a waterfall
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 17, 2011
Sequencing Facility	Joint Genomics Institute
Shotgun Sequencing Method	Sanger
Sequencer Used	Sanger ABI 3730
Read Type	Paired-end reads
Genome length (bp)	71547
Character of genome ends	Circularly Permuted
GC Content	56.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	R
Subcluster	--
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Candle MontyDev Nilo Papyrus Riparian Rope Send513 Weiss13 Yelo Zenon
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	less than 1 mm in diameter, clear, numerous. ( Morphology was uniform through all four assays.)
Morphotype	Siphoviridae
Number of Genes	98
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Send513_1 Send513_2 Send513_3 Send513_4 (terminase) Send513_5 Send513_6 (portal protein) Send513_7 Send513_8 Send513_9 Send513_10 Send513_11 (metalloprotease) Send513_12 (MuF-like minor capsid protein) Send513_13 Send513_14 Send513_15 Send513_16 Send513_17 Send513_18 Send513_19 Send513_20 Send513_21 Send513_22 Send513_23 Send513_24 Send513_25 (tail assembly chaperone) Send513_26 (tail assembly chaperone) Send513_27 (tape measure protein) Send513_28 Send513_29 Send513_30 Send513_31 (minor tail protein) Send513_32 Send513_33 Send513_34 Send513_35 (lysin A) Send513_36 Send513_37 Send513_38 Send513_39 (lysin B) Send513_40 (NrdH-like glutaredoxin) Send513_41 Send513_42 Send513_43 Send513_44 Send513_45 Send513_46 Send513_47 Send513_48 Send513_49 Send513_50 Send513_51 Send513_52 Send513_53 Send513_54 Send513_55 Send513_56 Send513_57 Send513_58 Send513_59 Send513_60 Send513_61 Send513_62 Send513_63 (O-methyltransferase) Send513_64 Send513_65 Send513_66 (DNA helicase) Send513_67 Send513_68 Send513_69 Send513_70 (RecA-like DNA recombinase) Send513_71 Send513_72 (DNA polymerase III sliding clamp) Send513_73 Send513_74 (DnaE-like DNA polymerase III) Send513_75 Send513_76 (thimidylate synthase) Send513_77 Send513_78 (band-7-like membrane protein) Send513_79 Send513_80 Send513_81 Send513_82 Send513_83 Send513_84 Send513_85 Send513_86 Send513_87 Send513_88 (endonuclease) Send513_89 (DNA primase/polymerase) Send513_90 Send513_91 Send513_92 (methyltransferase) Send513_93 Send513_94 Send513_95 Send513_96 Send513_97 Send513_98
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	JF704112
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2009GASUsend513AH
SEA Lysate Titer	1.68e11
Pitt Freezer Box#	3
Pitt Freezer Box Grid#	H12
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
Virtual Digest Picture	Download
EM Picture	Download

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