Gordonia phage Sprinklemunch
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Detailed Information for Phage Sprinklemunch
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByNikhita P. Kumar
Year Found2024
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.436944 N, 79.951389 W Map
Discovery NotesAashika Bhat, Megan Cook, Ethan Francilot

Sample was collected from a somewhat shady park with tree canopies casting shadows based on the time of day, which was roughly midday. The surrounding environment was a worn-down tree trunk that seemed to have been cut down and was slowly decomposing. The soil appeared dry since the air was humid regardless of the canopy shade from the trees surrounding. The sample was very loose and silty and a very dry tan shade of colour instead of what you would expect from a prototype of soil. There was a bit of difficulty filtering the soil but it was fairly easy to scoop into the phage buffer tube. The only problem was an abundance of little pebbles and mulch-like tree chips.
Naming NotesAll of our group members listed out favorite colors and combined them into one word.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2026
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NextSeq 1000
Read TypeSingle-end reads
Read Length100 bp
Approximate Shotgun Coverage113
Genome length (bp)51685
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTGCCAGCGGTA
GC Content66.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDW
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusBeing Annotated (Expected completion by 5/1/2026)
Number of Genes82
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 214
Pitt Freezer Box Grid# E4