Detailed Information for Phage TZGordon |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Tommy Zheng |
Year Found | 2019 |
Location Found | East Stroudsburg, PA USA |
Finding Institution | Lehigh University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | -4°C |
GPS Coordinates | 41.0633 N, 75.0603 W Map |
Discovery Notes | Soil was collected behind Tommy's family's restaurant. The soil was frozen; organic matter present in the soil(twigs/leaves). The soil was extremely watery/muddy. After finding Phage Gekyume(direct)on M.smegmatis, the soil was enriched with in a "cocktail" of 6 hosts and plated on individual lawns of different hosts. One host, Gordonia, was infected by this enrichment solution. The phage was purified by Meg Kenna. |
Naming Notes | The name merges the students initials with the host. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Aug 26, 2019 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 399 |
Genome length (bp) | 44904 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 12 bases |
Overhang Sequence | TCCGGGCCGGTA |
GC Content | 66.1% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | CU |
Subcluster | CU1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Lehigh University |
Annotation Status | In GenBank |
Plaque Notes | Large,very clear plaques, ranging from 3-5mm. |
Morphotype | Siphoviridae |
Number of Genes | 82 |
Number of tRNAs | 1 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MT553344 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
125 |
Pitt Freezer Box Grid# |
B2 |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |