| Detailed Information for Phage Triscuit |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Gabrielle Gentile |
| Year Found | 2016 |
| Location Found | Saint Clairsville, OH USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.082364 N, 80.920625 W Map |
| Discovery Notes | This sample was found in my backyard about a foot below the surface. The soil was dense and light tan in color. The weather was sunny, clear skies, and 76 degrees Fahrenheit. I collected my sample on 5/8/16 at around 2:30. |
| Naming Notes | This phage is named after the cracker. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 15, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2075 |
| Genome length (bp) | 67539 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3795 bp |
| GC Content | 58.3% |
| Sequencing Notes | Ends were determined by running Pause created by Texas A & M University, Center for Phage Technology |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | GG |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Triscuit forms clear plaques that are <1mm in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH047631 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
F4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
