Microbacterium phage Triscuit
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Detailed Information for Phage Triscuit
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByGabrielle Gentile
Year Found2016
Location FoundSaint Clairsville, OH USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.082364 N, 80.920625 W Map
Discovery NotesThis sample was found in my backyard about a foot below the surface. The soil was dense and light tan in color. The weather was sunny, clear skies, and 76 degrees Fahrenheit. I collected my sample on 5/8/16 at around 2:30.
Naming NotesThis phage is named after the cracker.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 15, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage2075
Genome length (bp)67539
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3795 bp
GC Content58.3%
Sequencing NotesEnds were determined by running Pause created by Texas A & M University, Center for Phage Technology
Fasta file available?Yes: Download fasta file
Characterization
ClusterGG
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesTriscuit forms clear plaques that are <1mm in size.
MorphotypeSiphoviridae
Number of Genes112
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Triscuit_1
(MerR-like helix-turn-helix DNA binding protein)

Triscuit_2

Triscuit_3

Triscuit_4

Triscuit_5

Triscuit_6

Triscuit_7

Triscuit_8
(MerR-like helix-turn-helix DNA binding protein)

Triscuit_9

Triscuit_10

Triscuit_11

Triscuit_12

Triscuit_13

Triscuit_14

Triscuit_15

Triscuit_16

Triscuit_17

Triscuit_18

Triscuit_19

Triscuit_20

Triscuit_21

Triscuit_22

Triscuit_23

Triscuit_24

Triscuit_25

Triscuit_26

Triscuit_27
(lysin A)

Triscuit_28

Triscuit_29

Triscuit_30

Triscuit_31

Triscuit_32
(phosphoesterase)

Triscuit_33

Triscuit_34
(terminase)

Triscuit_35
(portal protein)

Triscuit_36
(scaffolding protein)

Triscuit_37
(minor capsid protein)

Triscuit_38
(major capsid protein)

Triscuit_39

Triscuit_40
(helix-turn-helix DNA-binding domain protein)

Triscuit_41

Triscuit_42

Triscuit_43

Triscuit_44

Triscuit_45

Triscuit_46
(major tail subunit)

Triscuit_47

Triscuit_48

Triscuit_49
(tape measure protein)

Triscuit_50
(minor tail protein)

Triscuit_51
(minor tail protein)

Triscuit_52
(minor tail protein)

Triscuit_53

Triscuit_54

Triscuit_55

Triscuit_56

Triscuit_57

Triscuit_58
(DNA helicase)

Triscuit_59

Triscuit_60

Triscuit_61

Triscuit_62
(thymidylate kinase)

Triscuit_63
(MazG-like nucleotide pyrophosphohydrolase)

Triscuit_64

Triscuit_65

Triscuit_66
(thymidylate synthase)

Triscuit_67
(thymidylate synthase)

Triscuit_68

Triscuit_69
(deoxycytidylate deaminase)

Triscuit_70
(DNA polymerase I)

Triscuit_71

Triscuit_72

Triscuit_73

Triscuit_74

Triscuit_75

Triscuit_76

Triscuit_77

Triscuit_78
(RecB-like exonuclease/helicase)

Triscuit_79

Triscuit_80
(RecA-like DNA recombinase)

Triscuit_81

Triscuit_82
(holliday junction resolvase)

Triscuit_83

Triscuit_84

Triscuit_85

Triscuit_86

Triscuit_87 — tRNA
(tRNA-Asn(gtt))

Triscuit_88

Triscuit_89
(helix-turn-helix DNA-binding domain protein)

Triscuit_90

Triscuit_91

Triscuit_92

Triscuit_93

Triscuit_94
(RecA-like DNA recombinase)

Triscuit_95

Triscuit_96

Triscuit_97

Triscuit_98

Triscuit_99

Triscuit_100

Triscuit_101

Triscuit_102

Triscuit_103

Triscuit_104

Triscuit_105
(helix-turn-helix DNA-binding domain protein)

Triscuit_106
(MerR-like helix-turn-helix DNA binding protein)

Triscuit_107

Triscuit_108

Triscuit_109

Triscuit_110

Triscuit_111

Triscuit_112

Triscuit_113
(MerR-like helix-turn-helix DNA binding protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH047631
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 5
Pitt Freezer Box Grid# F4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload