| Detailed Information for Phage Typher | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | Paguette |
| Found By | Jhonny Rivera and Kayla Pimentel |
| Year Found | 2020 |
| Location Found | Amherst, MA USA |
| Finding Institution | University of Massachusetts Amherst |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.388912 N, 72.526712 W Map |
| Discovery Notes | After trying many times to get plaques from collecting dirt, we continued to get nothing after 22 plates. We proceeded to adopt a phage from a lab staff member in the lab department. She collected this phage from the dirt surrounding our nearby campus pond. It appears she had better luck than we did because we also tried to collect dirt from the campus pond and still got nothing. |
| Naming Notes | Paguette was our attempt to combine baguette and phage in a unique way. We then named our phage Typher after some debate. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3345 |
| Genome length (bp) | 41864 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EJ |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of Massachusetts Amherst |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are cloudy and appear to have a ring in the center of them. Consistent morphologies throughout the whole plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 60 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTypher_1 Typher_2 Typher_3 Typher_4 Typher_5 Typher_6 Typher_7 Typher_8 Typher_9 Typher_10 Typher_11 Typher_12 Typher_13 Typher_14 Typher_15 Typher_16 Typher_17 Typher_18 Typher_19 Typher_20 Typher_21 Typher_22 Typher_23 Typher_24 Typher_25 Typher_26 Typher_27 Typher_28 Typher_29 Typher_30 Typher_31 Typher_32 Typher_33 Typher_34 Typher_35 Typher_36 Typher_37 Typher_38 Typher_39 Typher_40 Typher_41 Typher_42 Typher_43 Typher_44 Typher_45 Typher_46 Typher_47 Typher_48 Typher_49 Typher_50 Typher_51 Typher_52 Typher_53 Typher_54 Typher_55 Typher_56 Typher_57 Typher_58 Typher_59 Typher_60 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON260821 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 124 |
| Pitt Freezer Box Grid# | B9 |
| Available Files | |
| GenBank File for Phamerator | Download |