| Detailed Information for Phage Virizion | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Ruotolo, Sarah and Sagalovsky, Alena |
| Year Found | 2024 |
| Location Found | Timonium, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 70°C |
| GPS Coordinates | 39.41758 N, 76.669872 W Map |
| Discovery Notes | The soil is very rich in organic matter due to it being collected near the bank of a stream, moist, collected slightly below surface level, the temperature at the time of collection was warm (73° F) but the stream water maintained a cooler temperature, thus allowing the soil to feel cooler as well. |
| Naming Notes | Named after a Grass/Fighting-type Legendary Pokémon |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 4, 2025 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Paired-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 6702 |
| Genome length (bp) | 69653 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Temperate phage S. achromogenes and S. bicolor host infection |
| Other Cluster Members | |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 12/4/2025) |
| Plaque Notes | Plaques - ~2 mm in diameter, clear, circular, clean edges, used -2 old lysate to make the -2 new plate on 10/15 TEM - Head length - 79nm Head width - 78nm Tail length (uncontracted) - 99nm Tail width (uncontracted) - 15nm Noted presence of whiskers |
| Morphotype | Siphoviridae |
| Number of Genes | 113 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewVirizion_1 Virizion_2 Virizion_3 Virizion_4 Virizion_5 Virizion_6 Virizion_7 Virizion_8 Virizion_9 Virizion_10 Virizion_11 Virizion_12 Virizion_13 Virizion_14 Virizion_15 Virizion_16 Virizion_17 Virizion_18 Virizion_19 Virizion_20 Virizion_21 Virizion_22 Virizion_23 Virizion_24 Virizion_25 Virizion_26 Virizion_27 Virizion_28 Virizion_29 Virizion_30 Virizion_31 Virizion_32 Virizion_33 Virizion_34 Virizion_35 Virizion_36 Virizion_37 Virizion_38 Virizion_39 Virizion_40 Virizion_41 Virizion_42 Virizion_43 Virizion_44 Virizion_45 Virizion_46 Virizion_47 Virizion_48 Virizion_49 Virizion_50 Virizion_51 Virizion_52 Virizion_53 Virizion_54 Virizion_55 Virizion_56 Virizion_57 Virizion_58 Virizion_59 Virizion_60 Virizion_61 Virizion_62 Virizion_63 Virizion_64 Virizion_65 Virizion_66 Virizion_67 Virizion_68 Virizion_69 Virizion_70 Virizion_71 Virizion_72 Virizion_73 Virizion_74 Virizion_75 Virizion_76 Virizion_77 Virizion_78 Virizion_79 Virizion_80 Virizion_81 Virizion_82 Virizion_83 Virizion_84 Virizion_85 Virizion_86 Virizion_87 Virizion_88 Virizion_89 Virizion_90 Virizion_91 Virizion_92 Virizion_93 Virizion_94 Virizion_95 Virizion_96 Virizion_97 Virizion_98 Virizion_99 Virizion_100 Virizion_101 Virizion_102 Virizion_103 Virizion_104 Virizion_105 Virizion_106 Virizion_107 Virizion_108 Virizion_109 Virizion_110 Virizion_111 Virizion_112 Virizion_113 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.1 x 10^6 pfu/mL |
| Pitt Freezer Box# | 212 |
| Pitt Freezer Box Grid# | D1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
