| Detailed Information for Phage Volt |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Olivia Bartholomew |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.44268 N, 79.956251 W Map |
| Discovery Notes | The soil sample was collected at 11:59 AM on 09/04/2017 one of the flower beds on the towers patio on the Forbes Avenue side. The soil is very rich and moist. The soil itself is dark brown and is very fine. The temperature outside is 77 degrees and it is sunny outside. The sample was transported in a bag for one hour and then was placed in a refrigerator overnight. |
| Naming Notes | I chose the name Volt for my phage, because it is strong and powerful name. Phages are strong and powerful so I thought the name was appropriate. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1302 |
| Genome length (bp) | 68553 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACTCTGCCCAT |
| GC Content | 50.2% |
| Sequencing Notes | 40% of the population contains a 118 bp deletion of 32984 - 33101 bp |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DP |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 150 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN813698 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
58 |
| Pitt Freezer Box Grid# |
B12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
