| Detailed Information for Phage Wheelbite | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Issac Aguayo |
| Year Found | 2015 |
| Location Found | Argyle, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.125786 N, 97.197407 W Map |
| Discovery Notes | The soil was taken on 1/26/2015 at 9:30 pm. The temperature was approximately 43 degrees Fahrenheit. The sample soil was taken 2 inches below the top of the soil, which was moist when I dug it up. There are not very many houses on that road because it is a residential road and the houses in the area are huge and have a lot of extra ground mainly populated by trees. There is some foot traffic down that road, one older woman who takes a power walk every day around 5 and an older man who walks his dad through the area, but the part of the road where I got the sample typically does not see a lot of people, only cars.Wildlife in the area consists of a rafter of turkeys that infrequently crosses the road, squirrels, birds and dogs that are not kept on leashes. |
| Naming Notes | The name "Wheelbite" comes from a term used by longboarders to describe when your deck comes in contact with one or more of your wheels and stops abruptly. It typically happens with a new set-up that has not yet been "dialed in". I picked this name because the only reason I knew of this road's existence was because the longboard club I joined last semester took me there to shred one day. Without them I would never have discovered this Phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 21, 2015 |
| Sequencing Facility | University of North Texas Health Science Center |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1369 |
| Sanger Finishing Reactions | 1 |
| Genome length (bp) | 59879 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AL |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques were typically circular, 2mm in diameter and completely clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewWheelbite_1 Wheelbite_2 Wheelbite_3 Wheelbite_4 Wheelbite_5 Wheelbite_6 Wheelbite_7 Wheelbite_8 Wheelbite_9 Wheelbite_10 Wheelbite_11 Wheelbite_12 Wheelbite_13 Wheelbite_14 Wheelbite_15 Wheelbite_16 Wheelbite_17 Wheelbite_18 Wheelbite_19 Wheelbite_20 Wheelbite_21 Wheelbite_22 Wheelbite_23 Wheelbite_24 Wheelbite_25 Wheelbite_26 Wheelbite_27 Wheelbite_28 Wheelbite_29 Wheelbite_30 Wheelbite_31 Wheelbite_32 Wheelbite_33 Wheelbite_34 Wheelbite_35 Wheelbite_36 Wheelbite_37 Wheelbite_38 Wheelbite_39 Wheelbite_40 Wheelbite_41 Wheelbite_42 Wheelbite_43 Wheelbite_44 Wheelbite_45 Wheelbite_46 Wheelbite_47 Wheelbite_48 Wheelbite_49 Wheelbite_50 Wheelbite_51 Wheelbite_52 Wheelbite_53 Wheelbite_54 Wheelbite_55 Wheelbite_56 Wheelbite_57 Wheelbite_58 Wheelbite_59 Wheelbite_60 Wheelbite_61 Wheelbite_62 Wheelbite_63 Wheelbite_64 Wheelbite_65 Wheelbite_66 Wheelbite_67 Wheelbite_68 Wheelbite_69 Wheelbite_70 Wheelbite_71 Wheelbite_72 Wheelbite_73 Wheelbite_74 Wheelbite_75 Wheelbite_76 Wheelbite_77 Wheelbite_78 Wheelbite_79 Wheelbite_80 Wheelbite_81 Wheelbite_82 Wheelbite_83 Wheelbite_84 Wheelbite_85 Wheelbite_86 Wheelbite_87 Wheelbite_88 Wheelbite_89 Wheelbite_90 Wheelbite_91 Wheelbite_92 Wheelbite_93 Wheelbite_94 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140434 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.4 x 10^7 pfu/mL |
| Date of SEA Lysate Titering | Mar 26, 2015 |
| Pitt Freezer Box# | 20 |
| Pitt Freezer Box Grid# | G3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |