| Detailed Information for Phage Wildcat |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | J. Falbo |
| Year Found | 2003 |
| Location Found | Latrobe , PA USA |
| Finding Institution | Latrobe High School |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.28113 N, 79.376446 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 8, 2006 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Sanger Sequencing |
| Genome length (bp) | 78296 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| GC Content | 57.2% |
| Sequencing Notes | July 28, 2014
Wildcat's 3' overhang is included at the left end of the genome.
This is because in the early days of mycobacteriophage sequencing, 3' overhangs were often reported at the left end of genomes. This is true for L5, D29, TM4, and others. At some point, we decided to put 3' overhangs at the right end because then you're representing one of the two real strands of DNA completely. But some phages got grandfathered in with left-end 3' overhangs, since their genomes were already submitted to GenBank by the time we made the switch.
That explains why Wildcat's overhang is at the left end. It doesn't explain why only 10/11ths of the overhang is at the left end. That seems to have been an error in creating the final fasta file. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | V |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 148 |
| Number of tRNAs | 22 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | DQ398052 |
| Refseq Number | NC_008206 |
| Published in Paper | Hatfull GF et al 2006, PLoS genetics: Exploring the mycobacteriophage metaproteome: phage genomics as an educational platform. |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
6 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
