| Detailed Information for Phage Yotsuba | 
	
	
		| Discovery Information | 
		| Isolation Host | Curtobacterium flaccumfaciens NRRL B-729 SEA | 
		
		
		
		
		| Found By | Sophia Sliter-Hays | 
		| Year Found | 2024 | 
		| Location Found | Pittsburgh, PA  United States | 
		| Finding Institution | University of Pittsburgh | 
		| Program | Phage Hunters Integrating Research and Education | 
		| From enriched soil sample? | Yes | 
		| Isolation Temperature | 30°C | 
		| GPS Coordinates | 40.455772 N, 79.9476 W  Map | 
		| Discovery Notes | In bushbed in front of 734 S Millvale Ave. 
 Annotated by Sophia Sliter-Hays.
 | 
		| Naming Notes | The titular character from the series by Kyohiko Azuma. | 
		
                
		| Sequencing Information | 
		| Sequencing Complete? | Yes | 
		| Date Sequencing Completed | Aug 13, 2024 | 
		| Sequencing Facility | Pittsburgh Bacteriophage Institute | 
		| Shotgun Sequencing Method | Illumina | 
		| Sequencer Used | Illumina NextSeq 1000 | 
		| Read Type | Single-end reads | 
		| Read Length | 100 bp | 
		| Approximate Shotgun Coverage | 3003 | 
		
		| Genome length (bp) | 57243 | 
		| Character of genome ends | 3' Sticky Overhang | 
		| Overhang Length | 9 bases | 
		| Overhang Sequence | CGCCCAGCC | 
		
		
		| GC Content | 59.5% | 
		
		| Fasta file available? | Yes: Download fasta file | 
		| Characterization | 
		| Cluster | JA | 
		| Subcluster | -- | 
                | Cluster Life Cycle | Unknown | 
		
		| Other Cluster Members |  | 
		| Annotating Institution | University of Pittsburgh | 
                | Annotation Status | In GenBank | 
		| Plaque Notes | The plaques are round, clear, and medium sized. | 
		
		| Number of Genes | 85 | 
		| Number of tRNAs | 0 | 
		| Number of tmRNAs | 0 | 
		| Has been Phamerated? | Yes | 
		| Gene List |  | 
		
		| Submitted Minimal DNA Master File | Download | 
		
		
		| Publication Info | 
		| Uploaded to GenBank? | Yes | 
		| GenBank Accession | PQ868965 | 
		| Refseq Number | None yet | 
		
        
        | Archiving Info | 
        
          | Archiving status | Archived | 
        
        
        
        
        
        | Pitt Freezer Box# | 220 | 
        
        
        
        | Pitt Freezer Box Grid# | E1 | 
        
        
		
		| Available Files | 
		
		
		
		
		
		
		| GenBank File for Phamerator | Download |