Gordonia phage Zangel
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| Detailed Information for Phage Zangel | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Angel Chen |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.444944 N, 79.959414 W Map |
| Discovery Notes | -The Phage was discovered in an location where there are plenty of foliage and plant life for a nutrient-rich environment for phage growth. -The weather was a 58 degrees Fahrenheit with cloudy skies and low wind. The surrounding environment had plenty of shrubbery with a large tree in its close proximity. The dirt condition was dry, slightly moist, and had a medium, slightly chunky consistency and cool to touch. -The soil sample was collected at 8:09am on 9/52017 with a transportation time of 15 minutes in the ziplock bag in a dark backpack to be stored in a room temperature cupboard for about 24 hours before being transported in a dark backpack to the lab on 9/6/2017 in a time span of 20 minutes. -After going through direct isolation and receiving a positive spot test result, plaque purification was carried out to isolate a singe phage and make a clonal population. |
| Naming Notes | The name came from the initial and first name of the person who discovered the phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | After incubating for 7 days at 30 degrees Celsius, there was no growth of a mesa. The plaques are consistently clear, indicating that Zangel is a lytic phage. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Incubated for two days at 30 degrees Celsius, the plaque appears as small pinpoints in the PYCa plates with a small blurry ring on the edges. The sizes are around 0-1mm and scattered more in the plates. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 118 ng/uL |
| Pitt Freezer Box# | 58 |
| Pitt Freezer Box Grid# | C5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |