| Detailed Information for Phage Ziko |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Anna Bondonese |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.443 N, 79.953 W Map |
| Discovery Notes | Ziko was found on 9/9/18 at 5:32 pm. The soil sample was taken from a planter in Schenley Park, Pittsburgh. The outside weather was 59 degrees Fahrenheit with winds blowing at 9 mph northeast. The soil was wet because it had been raining. Nearby vegetation included a few bushes. The soil was stored in a temperature controlled drawer until brought to lab. |
| Naming Notes | I have two dogs named Niko and Ziggy. For my phage name, I combined my dogs' names to make Ziko. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 469 |
| Genome length (bp) | 68860 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACTCTGCCCAT |
| GC Content | 50.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DP |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogen testing was performed and clear plaques were produced. Therefore, it can be concluded that Ziko is a lytic phage. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | This phage produces clear plaques that are almost circular, with some variation, measuring about 1-2 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 149 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK919478 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1x10^11 pfu/mL |
| Pitt Freezer Box# |
91 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
