| Detailed Information for Phage Ziko | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Anna Bondonese |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.443 N, 79.953 W Map |
| Discovery Notes | Ziko was found on 9/9/18 at 5:32 pm. The soil sample was taken from a planter in Schenley Park, Pittsburgh. The outside weather was 59 degrees Fahrenheit with winds blowing at 9 mph northeast. The soil was wet because it had been raining. Nearby vegetation included a few bushes. The soil was stored in a temperature controlled drawer until brought to lab. |
| Naming Notes | I have two dogs named Niko and Ziggy. For my phage name, I combined my dogs' names to make Ziko. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 469 |
| Genome length (bp) | 68860 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACTCTGCCCAT |
| GC Content | 50.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DP |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogen testing was performed and clear plaques were produced. Therefore, it can be concluded that Ziko is a lytic phage. |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | This phage produces clear plaques that are almost circular, with some variation, measuring about 1-2 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 149 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewZiko_1 Ziko_2 Ziko_3 Ziko_4 Ziko_5 Ziko_6 Ziko_7 Ziko_8 Ziko_9 Ziko_10 Ziko_11 Ziko_12 Ziko_13 Ziko_14 Ziko_15 Ziko_16 Ziko_17 Ziko_18 Ziko_19 Ziko_20 Ziko_21 Ziko_22 Ziko_23 Ziko_24 Ziko_25 Ziko_26 Ziko_27 Ziko_28 Ziko_29 Ziko_30 Ziko_31 Ziko_32 Ziko_33 Ziko_34 Ziko_35 Ziko_36 Ziko_37 Ziko_38 Ziko_39 Ziko_40 Ziko_41 Ziko_42 Ziko_43 Ziko_44 Ziko_45 Ziko_46 Ziko_47 Ziko_48 Ziko_49 Ziko_50 Ziko_51 Ziko_52 Ziko_53 Ziko_54 Ziko_55 Ziko_56 Ziko_57 Ziko_58 Ziko_59 Ziko_60 Ziko_61 Ziko_62 Ziko_63 Ziko_64 Ziko_65 Ziko_66 Ziko_67 Ziko_68 Ziko_69 Ziko_70 Ziko_71 Ziko_72 Ziko_73 Ziko_74 Ziko_75 Ziko_76 Ziko_77 Ziko_78 Ziko_79 Ziko_80 Ziko_81 Ziko_82 Ziko_83 Ziko_84 Ziko_85 Ziko_86 Ziko_87 Ziko_88 Ziko_89 Ziko_90 Ziko_91 Ziko_92 Ziko_93 Ziko_94 Ziko_95 Ziko_96 Ziko_97 Ziko_98 Ziko_99 Ziko_100 Ziko_101 Ziko_102 Ziko_103 Ziko_104 Ziko_105 Ziko_106 Ziko_107 Ziko_108 Ziko_109 Ziko_110 Ziko_111 Ziko_112 Ziko_113 Ziko_114 Ziko_115 Ziko_116 Ziko_117 Ziko_118 Ziko_119 Ziko_120 Ziko_121 Ziko_122 Ziko_123 Ziko_124 Ziko_125 Ziko_126 Ziko_127 — tRNA Ziko_128 — tRNA Ziko_129 Ziko_130 Ziko_131 Ziko_132 Ziko_133 Ziko_134 Ziko_135 Ziko_136 Ziko_137 Ziko_138 Ziko_139 Ziko_140 Ziko_141 Ziko_142 Ziko_143 Ziko_144 Ziko_145 Ziko_146 Ziko_147 Ziko_148 Ziko_149 Ziko_150 Ziko_151 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK919478 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1x10^11 pfu/mL |
| Pitt Freezer Box# | 91 |
| Pitt Freezer Box Grid# | E5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
