FurtherDiscovery: Protocols and Information
FurtherDiscovery
Once a genome is annotated, there are lots of comparative analyses and investigations to be done. Here we provide protocols for some addition experiments you can do.| Protocol Title | Description/Goal |
|---|---|
| Bred Main Protocol Details Download pdf |
Main protocol for performing BRED. |
| Lysogeny Experiments Details Download pdf |
Included in this protocol are the steps used to make a lysogen, verify a lysogen, determine the efficiency of lysogeny , and test the lysogen (immunity assays). |
| Additional Hosts Details Download pdf |
To expand Phagehunting to hosts that are related to M. smegmatis mc2 155. |
| Growing Arthrobacter Details Download pdf |
This protocol will refer to the protocols for finding phages on M. smegmatis mc2 155. |
| Exploring Bacteriophage Biology Details Download pdf |
This document is a description of analyses that you and your students can do to investigate your phage genomes. |
| BRED Protocol: Bacteriophage Recombineering with Electroporated DNA Details Download pdf |
This system is used to generate mutations in lytically replicating mycobacteriophages. |