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FurtherDiscovery: Protocols and Information
FurtherDiscovery

FurtherDiscovery

Once a genome is annotated, there are lots of comparative analyses and investigations to be done. Here we provide protocols for some addition experiments you can do.
Protocol TitleDescription/Goal
Bred Main Protocol
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Main protocol for performing BRED.
Lysogeny Experiments
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Included in this protocol are the steps used to make a lysogen, verify a lysogen, determine the efficiency of lysogeny , and test the lysogen (immunity assays).
Additional Hosts
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To expand Phagehunting to hosts that are related to M. smegmatis mc2 155.
Growing Arthrobacter
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This protocol will refer to the protocols for finding phages on M. smegmatis mc2 155.
Exploring Bacteriophage Biology
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This document is a description of analyses that you and your students can do to investigate your phage genomes.
BRED Protocol: Bacteriophage Recombineering with Electroporated DNA
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This system is used to generate mutations in lytically replicating mycobacteriophages.