Preparation: Protocols and Information
Preparation
Protocols and instructions to get set up to begin phagehunting. Includes information on how/where to order lab supplies, how to make media, safety precautions, naming guidelines, etc.| Protocol Title | Description/Goal |
|---|---|
| Preparing Working Media Details Download pdf |
Finish preparing several common reagents (Phage Buffer, 7H9, MBTA) by adding final ingredients. |
| Preparing ADC Details Download pdf |
Prepare ADC for use as the energy source for mycobacterial growth in 7H9 cultures or 7H10 plates. |
| Preparing Phage Buffer Details Download pdf |
Prepare standard buffer for storing and diluting mycobacteriophages. |
| Preparing 7H9 Details Download pdf |
Prepare the base broth for use in growing mycobacterial cultures and plating mycobacterial lawns. |
| Ordering Information for Common Supplies Details Download pdf |
This protocol contains the vendors from which we order the most common supplies, as well as the catalog numbers of those items. |
| M. smegmatis Media Additions Details Download pdf |
A reference table for the amount of additives to be added to media of M. smegmatis, as well as markers’ colors to denote their presence. |
| Preparing 0.2% Glucose Agar Plates Details Download pdf |
Prepare 0.2% glucose agar plates for growing M. smegmatis. |
| Preparing MBTA Details Download pdf |
Prepare MBTA stock for later use in plating bacterial lawns. |
| Growing Mycobacterium smegmatis mc2 155 Details Download pdf |
To grow and maintain a plate stock of M. smegmatis mc2155, obtain a smooth starter culture for amplification using Tween®80, and grow bacteria for experiments. |
| Growing Mycobacterium smegmatis stlr 02 Details Download pdf |
To grow and maintain a plate stock of M. smegmatis stlr 02, obtain a smooth starter culture for amplification using Tween®80, and grow bacteria for experiments. |