Gordonia phage Angelique
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Detailed Information for Phage Angelique
Discovery Information
Isolation HostGordonia terrae 3612
Found ByAngelique Ogunsemowo
Year Found2016
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature32°C
GPS Coordinates40.4439 N, 79.9575 W Map
Discovery Notes-Weather conditions at time of soil collection: 72 degrees Farenheit, slight breeze
-Sample was transported for about 15 minutes prior to storage
-Soil conditions: moist, rich in mulch and plant debris
Naming NotesThis phage is the name of the founder.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1548
Genome length (bp)50970
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGTATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCY
SubclusterCY1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPhage Angelique was grown on the bacteria Gordonia terrae on a PYCa growth media, and incubated at 30°C for about 2 days.
Angelique produces round, “halo” looking plaques that can range in size from approximately 2-3mm. 
Has been Phamerated?Yes
Gene List

Click to View

Angelique_1
(terminase small subunit)

Angelique_2
(terminase large subunit)

Angelique_3
(portal protein)

Angelique_4
(capsid maturation protease)

Angelique_5
(scaffolding protein)

Angelique_6
(capsid decoration protein)

Angelique_7
(major capsid protein)

Angelique_8

Angelique_9
(head-to-tail connector complex protein)

Angelique_10
(head-to-tail connector complex protein)

Angelique_11

Angelique_12

Angelique_13
(head-to-tail connector complex protein)

Angelique_14
(major tail protein)

Angelique_15
(tail assembly chaperone)

Angelique_16
(tail assembly chaperone)

Angelique_17

Angelique_18
(tape measure protein)

Angelique_19
(minor tail protein)

Angelique_20
(minor tail protein)

Angelique_21

Angelique_22
(lysin A, protease M15 domain)

Angelique_23
(lysin A, glycosyl hydrolase domain)

Angelique_24
(membrane protein)

Angelique_25

Angelique_26

Angelique_27
(membrane protein)

Angelique_28
(lysin B)

Angelique_29
(minor tail protein)

Angelique_30
(minor tail protein)

Angelique_31

Angelique_32

Angelique_33
(minor tail protein)

Angelique_34

Angelique_35

Angelique_36

Angelique_37
(helix-turn-helix DNA binding protein)

Angelique_38

Angelique_39
(integrase)

Angelique_40
(helix-turn-helix DNA binding domain protein)

Angelique_41
(peptidase)

Angelique_42

Angelique_43
(methyltransferase)

Angelique_44
(immunity repressor)

Angelique_45
(helix-turn-helix DNA binding domain protein)

Angelique_46

Angelique_47

Angelique_48
(antirepressor)

Angelique_49

Angelique_50

Angelique_51

Angelique_52

Angelique_53

Angelique_54

Angelique_55
(exonuclease)

Angelique_56
(ssDNA binding protein)

Angelique_57

Angelique_58

Angelique_59
(DNA methylase)

Angelique_60

Angelique_61

Angelique_62

Angelique_63

Angelique_64

Angelique_65

Angelique_66

Angelique_67

Angelique_68

Angelique_69
(RusA-like resolvase)

Angelique_70

Angelique_71

Angelique_72

Angelique_73

Angelique_74

Angelique_75

Angelique_76
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH651167
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3 x 10^11 pfu/ml
Date of SEA Lysate Titering Oct 10, 2016
Pitt Freezer Box# 42
Pitt Freezer Box Grid# B3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload