Gordonia phage Angelique
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Detailed Information for Phage Angelique
Discovery Information
Isolation HostGordonia terrae 3612
Found ByAngelique Ogunsemowo
Year Found2016
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature32°C
GPS Coordinates40.4439 N, 79.9575 W Map
Discovery Notes-Weather conditions at time of soil collection: 72 degrees Farenheit, slight breeze
-Sample was transported for about 15 minutes prior to storage
-Soil conditions: moist, rich in mulch and plant debris
Naming NotesThis phage is the name of the founder.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1548
Genome length (bp)50970
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGTATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCY
SubclusterCY1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusAwaiting_QC
Plaque NotesPhage Angelique was grown on the bacteria Gordonia terrae on a PYCa growth media, and incubated at 30°C for about 2 days.
Angelique produces round, “halo” looking plaques that can range in size from approximately 2-3mm. 
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3 x 10^11 pfu/ml
Date of SEA Lysate Titering Oct 10, 2016
Pitt Freezer Box# 42
Pitt Freezer Box Grid# B3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload