| Detailed Information for Phage Christian | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Jewel Smith |
| Year Found | 2015 |
| Location Found | West Deptford, NJ USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.8318 N, 75.1896 W Map |
| Discovery Notes | This soil sample was taken from under a tree at the park at my apartment complex. |
| Naming Notes | This soil sample was dug up from under a tree at the park with the assistance of my nephew Christian. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1818 |
| Genome length (bp) | 43081 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | GGTAACCGTGATA |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlbanese Aledel AppleCider AustinPowers Beethoven Bennie BigMack Bodacious BrotherBLo CallieOMalley Canowicakte Carpal ChewChew Cholula Chridison Christian CristinaYang Daiboju Dino DreamTeam DrRobert EstebanJulior Eunoia Fluke Gisselle Glenn GreenHearts Greenhouse HeadNerd Herb Huckleberry HunterDalle Huntingdon Immaculata Joann Jumboset Kalizoi KingBob Kittykat Korra Lakshmi Lasagna Lennox LilStuart Litotes Lucy Makoto MamaPearl Maria1952 MeganNoll Misaeng Moki MrGloopy Nancia Nubia OMalley OurGirlNessie Oxynfrius PartyCup PinkFriday PitaDog Potatoes Preamble Pterodactyl Pumancara RAP15 RcigaStruga Riovina Riverdale Rozby Savage2526 Scuttle Sergei Supakev Suppi TattModd Temper16 Urla Vallejo Vulture Wawa Wayne WonderBoy Zorro |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | With various Calcium concentrations experimented with, it was found that at room temperature and overnight incubation there are tiny but plentiful and clear plaques grown. At 30 degrees Celsius and overnight incubation there were bigger plaques but still small and clear but less plentiful. At 37 degrees Celsius and overnight incubation there is no growth at all. As the concentration of calcium increases the bigger the plaques grow. Calcium concentrations experimented with were: 0 mM, 5 mM, 25 mM, and 50 mM. At 30 degrees Celsius without calcium there is no growth. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewChristian_1 Christian_2 Christian_3 Christian_4 Christian_5 Christian_6 Christian_7 Christian_8 Christian_9 Christian_10 Christian_11 Christian_12 Christian_13 Christian_14 Christian_15 Christian_16 Christian_17 Christian_18 Christian_19 Christian_20 Christian_21 Christian_22 Christian_23 Christian_24 Christian_25 Christian_26 Christian_27 Christian_28 Christian_29 Christian_30 Christian_31 Christian_32 Christian_33 Christian_34 Christian_35 Christian_36 Christian_37 Christian_38 Christian_39 Christian_40 Christian_41 Christian_42 Christian_43 Christian_44 Christian_45 Christian_46 Christian_47 Christian_48 Christian_49 Christian_50 Christian_51 Christian_52 Christian_53 Christian_54 Christian_55 Christian_56 Christian_57 Christian_58 Christian_59 Christian_60 Christian_61 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140404 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Cabrini College |
| SEA Lysate Titer | 7.8 X 10^6 |
| Date of SEA Lysate Titering | Jul 1, 2015 |
| Pitt Freezer Box# | 149 |
| Pitt Freezer Box Grid# | B12 |
| Available Files | |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |