Arthrobacter phage Lucy
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Detailed Information for Phage Lucy
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByKate Starr
Year Found2015
Location FoundImmaculata, PA United States
Finding InstitutionCabrini University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.013017 N, 75.605443 W Map
Discovery NotesSoil sample was retrieved from under a tree and taken from a whole dug by a dog.
Naming NotesLucy is the name of the dog that dug the hole where the soil sample was taken from.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 17, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1947
Genome length (bp)42944
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceGGTAACCGTGATA
GC Content60.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAK
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesLucy produces small, clear, round plaques. Plaque size remained consistent during the isolation process. Lucy has a fairly low titer. Optimal growth occurs between room temperature and 30 degrees Celsius. Does not grow at 37 degrees Celsius. Growth occurred with 0, 22.5, and 50 mM CaCl2 top agar. Also, Lucy grows better with MgSO4 rather than CaCl2. The plaques were bigger, and more robust in the varying conditions of MgSO4. The MgSO4 concentrations used were: 5, 25, and 50 mM.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX576641
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator Cabrini College
SEA Lysate Titer 2.4E7
Date of SEA Lysate Titering Jul 20, 2015
Pitt Freezer Box# 2
Pitt Freezer Box Grid# F4
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload