| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | JAST16 |
| Found By | S.Mendez, T.Feroz, A.Ornn, J.Hudson |
| Year Found | 2022 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.465 N, 94.727778 W Map |
| Discovery Notes | The soil sample was collected at LeTourneau University in Longview, Texas, USA outside of the 53,000-square-foot Glaske Center for Engineering, Science, and Technology building on 23rd August 2022 around 5:00 p.m. CST. The ambient temperature was 28.3 °C and the weather was mostly cloudy. The soil was moist because it had rained the night before, about 2.05 in. |
| Naming Notes | The former name of our phage was JAST16, that was derived by taking the first name initials of each member of our research team, and 16 for our sample number allocated by the instructor during the enrichment isolation process of various phages. However, the “JAST16” name was not accepted, so we changed it to Glaske16. Since the sample was collected in front of the Glaske building and 16 was our sample number, we decided to rename it as Glaske16. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 360 |
| Genome length (bp) | 81156 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | The phage exhibited clear plaques with the average diameter of 1.7 mm (range 1.0 - 2.0 mm, n= 3). The average distance between plaques picked during the phage purification process was 1. 5cm. The plaques were generally round and uniform across the entire plate. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553909 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.9 x 10^9 pfu/ml |
| Pitt Freezer Box# |
150 |
| Pitt Freezer Box Grid# |
D11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
