| Detailed Information for Phage MelBee03 |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Madelyn Brookins |
| Year Found | 2021 |
| Location Found | Hudson, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 29°C |
| GPS Coordinates | 44.984533 N, 92.7545 W Map |
| Discovery Notes | Found in Hudson,WI from a compost pile next to West side of a garage, in a alley way.
Adopted from Karen Klyczek |
| Naming Notes | I chose the name MelBee03. MelBee03, comes from a singer that is a judge on American Idol. Her name is actually spelled Mel B, but I thought it would be fun to add bee to it. And 03 rhymes with bee and 03 is my birth date. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 23, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 99 |
| Genome length (bp) | 17441 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | They are all the same size, the plates are not cloudy and can see the plaques just fine. None of my plaques had halos, until lysate titer. |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP580518 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
140 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
