Microbacterium phage Minima
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Detailed Information for Phage Minima
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByDathan Stone
Year Found2017
Location FoundGuilford, CT United States
Finding InstitutionSouthern Connecticut State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates41.3172 N, 72.7209 W Map
Discovery NotesThe sample was found in the morning, 70F and the soil was a mix of dry and wet. About 10mm was dug down, and grass was pulled to mix in with the soil sample. Since microbacterium foliorum was discovered in the phylosphere of grasses, I wanted to mix some of the grass into the soil.
Naming Notes"Minima" is Latin for "tiny". Minima produces 1mm plaques that look like archery targets.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage9882
Genome length (bp)17362
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCCCGCCCCA
GC Content68.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEE
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusAwaiting_QC
Plaque NotesThe plaque is 1mm, and has a small clear center, with a noticeable fuzzy ring around it. This resembles a bullseye target and is a temperate phage.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.7 x 10^10
Pitt Freezer Box# 72
Pitt Freezer Box Grid# B5
Available Files
Plaque PictureDownload
EM PictureDownload