| Detailed Information for Phage Belthelas | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Hope Kirby, Hannah Wilson |
| Year Found | 2018 |
| Location Found | Oxford, OH USA |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.514 N, 84.7261 W Map |
| Discovery Notes | It was found from lichen near the base of a tree about thirty feet away from a creek. It was near undeveloped hiking trails. The tree was about 2 feet thick, it was humid, and there were fungi and decomposing leaves near the bottom as well but on the ground. |
| Naming Notes | Belthelas= Belthor + Athelas Belthor: Elvish for Killer Athelas: powerful herb in Lord of the Rings used by Aragorn to heal Eowyn and Faramir of the dark powers found in pure evil. It was thought to be useless until the king came and called for it. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3320 |
| Genome length (bp) | 17502 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 68.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba |
| Annotating Institution | Miami University |
| Annotation Status | In GenBank |
| Plaque Notes | - Started out as large halo, but shrunk with time until the turbid halo was only about a twentieth of the plaque. There did not appear to be any elevation. - Most started out relatively large, but with time also the plaques became smaller as more bacteria was killed |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBelthelas_1 Belthelas_2 Belthelas_3 Belthelas_4 Belthelas_5 Belthelas_6 Belthelas_7 Belthelas_8 Belthelas_9 Belthelas_10 Belthelas_11 Belthelas_12 Belthelas_13 Belthelas_14 Belthelas_15 Belthelas_16 Belthelas_17 Belthelas_18 Belthelas_19 Belthelas_20 Belthelas_21 Belthelas_22 Belthelas_23 Belthelas_24 Belthelas_25 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK801730 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.86x10^11 |
| Pitt Freezer Box# | 74 |
| Pitt Freezer Box Grid# | C6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
