| Detailed Information for Phage Niffler | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Hannah Jaycocks |
| Year Found | 2022 |
| Location Found | Stuart, FL United States |
| Finding Institution | Indian River State College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 27.110283 N, 80.256793 W Map |
| Discovery Notes | Found under a shady tree on a dry, sunny day in Halpatiokee Park, right off of I-95 in south Florida, surface level soil was collected. Tree roots and small berries were present at the collection site, and the soil was drier than it was moist. |
| Naming Notes | As a dedication to my mom, the phage is named after her favorite character from the Fantastic Beasts franchise. A Niffler is a magical beast that has a long snout, with fluffy black fur and is attracted to shiny things. This makes a Niffler a great treasure hunter, but beware, as these harmless creatures wreak havoc if set loose indoors. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1769 |
| Genome length (bp) | 17023 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGCCCCA |
| GC Content | 69.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAlucard Anseraureola Aries55 Astro16 Avvas Azizam Aztec Baine BarBear Belthelas BinkBonk Bintii Biscayne Bodhi17 BonaeVitae BoomRoasted Bradley2 Bri160 BrokMonster Bullzi2019 Burgy BurtonThePup Cayde6 Cazares Charbie Chatham Cheesecake Ciel Clownery Concrete DaftyDuck Danimal Danno Dongwon Dooby Douggie EdElric Efeko Eightball Ekko14 Elspeth Erin Gabbspett Gardevoir Gillker Godfather GoldenBlanche Goobery HarperAnne Hernandez44 Horacetta Hulk Jahseh JaimeB Jannah Jerky JoBros JooneeDee Josuke JRok Kaijohn KayPaulus Kevanna KillerTomato Kojax4 KyriosFaba LaviMo Leafy LemonPepper LionelHutz Livingwater Loca Lola20 Lunatic Luxx Majesty ManAs Maneater MasterPitts McShie MelBee03 Miaurora Minima MirimarMandM MrGreen Naby Namsahir NeumannU Niffler Nobel Noelani Oaklynn Octopus Otwor Owens PaoPu Picnic Pondwater PoRanda Publix Puddles3 PuffyCat Quaker Rhogar Rhysand RoscoWindex Sage7 SantasSleigh Sara Scamander Scrunchy SeaWolves Sippinontea Slentz Slizam Snuffles Stem TeddyBoy TimoTea TinyTruffula TomBrady12 Trireme Upsilon Vanisius VitulaEligans Witch Wolfpack Yami Yasuo YertPhresh Yubaba |
| Annotating Institution | Indian River State College |
| Annotation Status | In GenBank |
| Plaque Notes | Plagues are circular in shape, slightly clustered, and have consistency in appearance. Plaques are completely lysed, and are observed in occasional clusters throughout the plate. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewNiffler_1 Niffler_2 Niffler_3 Niffler_4 Niffler_5 Niffler_6 Niffler_7 Niffler_8 Niffler_9 Niffler_10 Niffler_11 Niffler_12 Niffler_13 Niffler_14 Niffler_15 Niffler_16 Niffler_17 Niffler_18 Niffler_19 Niffler_20 Niffler_21 Niffler_22 Niffler_23 Niffler_24 Niffler_25 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP059643 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 169 |
| Pitt Freezer Box Grid# | C9 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
