Detailed Information for Phage Oaker |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Danielle DeNigris |
Year Found | 2012 |
Location Found | Philadelphia, PA USA |
Finding Institution | Saint Joseph's University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 39.971361 N, 75.194733 W Map |
Discovery Notes | Soil taken from gazelle exhibit at the Philadelphia Zoo. Soil was in a shady area near kopje and was 2-2.5 cm deep. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 19, 2013 |
Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
Shotgun Sequencing Method | 454 Pyrosequencing |
Approximate Shotgun Coverage | 250 |
Genome length (bp) | 69099 |
Character of genome ends | Circularly Permuted |
GC Content | 57.5% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | H |
Subcluster | H1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | The plaques were fast growing appearing after about 28 hours but not as soon as 24 hours. The plaques were small (about 1 mm in diameter) and very clear. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KF986247 |
Refseq Number | NC_023578 |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2012STJOoakerDMD |
SEA Lysate Titer |
6.1 x 10^10 pfu/mL |
Date of SEA Lysate Titering |
Oct 18, 2012 |
Pitt Freezer Box# |
39 |
Pitt Freezer Box Grid# |
37 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |