| Detailed Information for Phage Phreeze |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rina Watanabe |
| Year Found | 2017 |
| Location Found | Los Angeles, CA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.04604 N, 118.29136 W Map |
| Discovery Notes | Phage Phreeze was isolated from moist soil collected on 10/08/17 in Korea Town (Los Angeles) by Rina Watanabe. Air temperature at time of collection was 20°C. Phreeze was isolated by direct plating at 30°C.
Characterization was performed by Keith Mitchell.
Midi-Lysate (Archive) Titer: 8.25*10^12 PFU/mL
Date of High-Titer Lysate Titration: 11/29/17
MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker |
| Naming Notes | Named after course instructor Dr. Amanda Freise. Both the name of the instructor and the name of the phage are pronounced "freeze." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 2, 2018 |
| Genome length (bp) | 67906 |
| Character of genome ends | Unknown |
| GC Content | 57.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | H |
| Subcluster | H1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | small turbid plaques, 0.5mm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN010756 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
58 |
| Pitt Freezer Box Grid# |
F6 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
