Mycobacterium phage Phreeze
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Detailed Information for Phage Phreeze
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRina Watanabe
Year Found2017
Location FoundLos Angeles, CA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates34.04604 N, 118.29136 W Map
Discovery NotesPhage Phreeze was isolated from moist soil collected on 10/08/17 in Korea Town (Los Angeles) by Rina Watanabe. Air temperature at time of collection was 20°C. Phreeze was isolated by direct plating at 30°C.

Characterization was performed by Keith Mitchell.

Midi-Lysate (Archive) Titer: 8.25*10^12 PFU/mL
Date of High-Titer Lysate Titration: 11/29/17

MIMG103AL-F17 Instructor: Dr. Amanda Freise
Teaching Assistant: Andrew Lund
Undergraduate Assistant: Keith Mitchell
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
Naming NotesNamed after course instructor Dr. Amanda Freise. Both the name of the instructor and the name of the phage are pronounced "freeze."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 2, 2018
Genome length (bp)67906
Character of genome endsUnknown
GC Content57.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterH
SubclusterH1
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 12/21/2018)
Plaque Notessmall turbid plaques, 0.5mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 58
Pitt Freezer Box Grid# F6
Available Files
Plaque PictureDownload
EM PictureDownload