| Detailed Information for Phage Shinji | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Rowen, Matthew, Jaden, Peter |
| Year Found | 2022 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.441732 N, 79.960117 W Map |
| Discovery Notes | Discovered in a patch of soil outside a walkway. Soil was damp and had a lot of natural material (grass, sticks, small rocks) in it. Sample was taken from the surface. |
| Naming Notes | In 2015, fifteen years after a global cataclysm known as the Second Impact, teenager Shinji Ikari is summoned to the futuristic city of Tokyo-3 by his estranged father Gendo Ikari, director of the special paramilitary force Nerv. Shinji witnesses United Nations forces battling an Angel, one of a race of giant monstrous beings whose awakening was foretold by the Dead Sea Scrolls. Because of the Angels' near-impenetrable force-fields, Nerv's giant Evangelion bio-machines, synchronized to the nervous systems of their pilots and possessing their own force-fields, are the only weapons capable of keeping the Angels from annihilating humanity. Nerv officer Misato Katsuragi escorts Shinji into the Nerv complex beneath the city, where his father pressures him into piloting the Evangelion Unit-01 against the Angel. Without training, Shinji is quickly overwhelmed in the battle, causing the Evangelion to go berserk and savagely kill the Angel on its own. Following hospitalization, Shinji moves in with Misato and settles into life in Tokyo-3. In his second battle, Shinji destroys an Angel but runs away afterward, distraught. Misato confronts Shinji and he decides to remain a pilot. The Nerv crew and Shinji must then battle and defeat the remaining fourteen Angels to prevent the Third Impact, a global cataclysm that would destroy the world. Evangelion Unit-00 is repaired shortly afterward. Shinji tries to befriend its pilot, the mysterious, socially isolated teenage girl Rei Ayanami. With Rei's help, Shinji defeats another Angel. They are then joined by the pilot of Evangelion Unit-02, the multitalented but insufferable teenager Asuka Langley Sōryu, who is German-Japanese-American. Together, the three of them manage to defeat several Angels. As Shinji adjusts to his new role as a pilot, he gradually becomes more confident and self-assured. Asuka moves in with Shinji, and they begin to develop confusing feelings for one another, kissing at her provocation. After being absorbed by an Angel, Shinji breaks free thanks to Eva acting on its own. He is later forced to fight an infected Evangelion Unit-03 and watches its pilot, his friend and classmate Toji Suzuhara, become incapacitated and permanently disabled. Asuka loses her self-confidence following a defeat and spirals into depression. This is worsened by her next fight, against an Angel which attacks her mind and forces her to relive her worst fears and childhood trauma, resulting in a mental breakdown. In the next battle, Rei self-destructs Unit-00 and dies to save Shinji's life. Misato and Shinji visit the hospital where they find Rei alive but claiming she is "the third Rei". Misato forces scientist Ritsuko Akagi to reveal the dark secrets of Nerv, the Evangelion boneyard, and the dummy plug system which operates using clones of Rei, who was herself created with the DNA of Shinji's mother, Yui Ikari. This succession of events leaves Shinji emotionally scarred and alienated from the rest of the characters. Kaworu Nagisa replaces the catatonic Asuka as the pilot of Unit-02. Kaworu, who initially befriends Shinji and gains his trust, is in truth the final foretold Angel, Tabris. Kaworu fights Shinji, then realizes that he must die if humanity is to survive and asks Shinji to kill him. Shinji hesitates but eventually kills Kaworu; the event makes Shinji overridden with guilt. After the final Angel is defeated, Seele, the mysterious cabal overseeing the events of the series, triggers the "Human Instrumentality Project", a forced evolution of humanity in which the souls of all mankind are merged for benevolent purposes, believing that if unified, humanity could finally overcome the loneliness and alienation that has eternally plagued mankind. Shinji's soul grapples with the reason for his existence and reaches an epiphany that he needs others to thrive, enabling him to destroy the wall of negative emotions that torment him and reunite with the others, who congratulate him. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 537 |
| Genome length (bp) | 58541 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DC |
| Subcluster | DC1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewArri (DC1) Bakery (DC1) Barb (DC1) ClamChowder (DC1) Danyall (DC1) Evamon (DC1) Fireball (DC1) Fugax (DC1) Gezellig (DC1) Halo3 (DC1) Jambalaya (DC1) KimmyK (DC1) Mutzi (DC1) Nubi (DC1) Phlop (DC1) PinkCoffee (DC1) Portcullis (DC1) PullumCavea (DC1) RogerDodger (DC1) Salvador (DC1) Savbucketdawg (DC1) Shinji (DC1) SmokingBunny (DC1) TillyBobJoe (DC1) Togo (DC1) Twister6 (DC1) Valary (DC1) VanDeWege (DC1) Wizard (DC1) YungMoney (DC1) Clown (DC2) |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | Overdue (Expected completion by 3/5/2024) |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewShinji_Draft_1 Shinji_Draft_2 Shinji_Draft_3 Shinji_Draft_4 Shinji_Draft_5 Shinji_Draft_6 Shinji_Draft_7 Shinji_Draft_8 Shinji_Draft_9 Shinji_Draft_10 Shinji_Draft_11 Shinji_Draft_12 Shinji_Draft_13 Shinji_Draft_14 Shinji_Draft_15 Shinji_Draft_16 Shinji_Draft_17 Shinji_Draft_18 Shinji_Draft_19 Shinji_Draft_20 Shinji_Draft_21 Shinji_Draft_22 Shinji_Draft_23 Shinji_Draft_24 Shinji_Draft_25 Shinji_Draft_26 Shinji_Draft_27 Shinji_Draft_28 Shinji_Draft_29 Shinji_Draft_30 Shinji_Draft_31 Shinji_Draft_32 Shinji_Draft_33 Shinji_Draft_34 Shinji_Draft_35 Shinji_Draft_36 Shinji_Draft_37 Shinji_Draft_38 Shinji_Draft_39 Shinji_Draft_40 Shinji_Draft_41 Shinji_Draft_42 Shinji_Draft_43 Shinji_Draft_44 Shinji_Draft_45 Shinji_Draft_46 Shinji_Draft_47 Shinji_Draft_48 Shinji_Draft_49 Shinji_Draft_50 Shinji_Draft_51 Shinji_Draft_52 Shinji_Draft_53 Shinji_Draft_54 Shinji_Draft_55 Shinji_Draft_56 Shinji_Draft_57 Shinji_Draft_58 Shinji_Draft_59 Shinji_Draft_60 Shinji_Draft_61 Shinji_Draft_62 Shinji_Draft_63 Shinji_Draft_64 Shinji_Draft_65 Shinji_Draft_66 Shinji_Draft_67 Shinji_Draft_68 Shinji_Draft_69 Shinji_Draft_70 Shinji_Draft_71 Shinji_Draft_72 Shinji_Draft_73 Shinji_Draft_74 Shinji_Draft_75 Shinji_Draft_76 Shinji_Draft_77 Shinji_Draft_78 Shinji_Draft_79 Shinji_Draft_80 Shinji_Draft_81 Shinji_Draft_82 Shinji_Draft_83 Shinji_Draft_84 Shinji_Draft_85 Shinji_Draft_86 Shinji_Draft_87 Shinji_Draft_88 Shinji_Draft_89 Shinji_Draft_90 Shinji_Draft_91 Shinji_Draft_92 Shinji_Draft_93 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 159 |
| Pitt Freezer Box Grid# | G12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
