| Detailed Information for Phage Widow | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Former names | spider-bush |
| Found By | Emelia Tremblay, Griffin Lawrence |
| Year Found | 2021 |
| Location Found | Mattapoisett, MA United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 24°C |
| GPS Coordinates | 41.658733 N, 70.805472 W Map |
| Discovery Notes | While 16 soil samples from Maine did not produce phage, the first Massachusetts soil sample contained the phage Widow. |
| Naming Notes | Name was chosen due to the live spider found in the dirt sample widow was isolated from. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1328 |
| Genome length (bp) | 43656 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCCGCCGGTCT |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CD |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Widow Forms a stable lysogen Efficiency of lysogeny 26.56% |
| Other Cluster Members | |
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | Widow produces plaques of varying sizes and of varying clarity depending on the nutrient level it finds itself in. We find that on average the plaques produce halos. It is important to note that have Widows plaques were consistently non-circular. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewWidow_1 Widow_2 Widow_3 Widow_4 Widow_5 Widow_6 Widow_7 Widow_8 Widow_9 Widow_10 Widow_11 Widow_12 Widow_13 Widow_14 Widow_15 Widow_16 Widow_17 Widow_18 Widow_19 Widow_20 Widow_21 Widow_22 Widow_23 Widow_24 Widow_25 Widow_26 Widow_27 Widow_28 Widow_29 Widow_30 Widow_31 Widow_32 Widow_33 Widow_34 Widow_35 Widow_36 Widow_37 Widow_38 Widow_39 Widow_40 Widow_41 Widow_42 Widow_43 Widow_44 Widow_45 Widow_46 Widow_47 Widow_48 Widow_49 Widow_50 Widow_51 Widow_52 Widow_53 Widow_54 Widow_55 Widow_56 Widow_57 Widow_58 Widow_59 Widow_60 Widow_61 Widow_62 Widow_63 Widow_64 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON456352 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 142 |
| Pitt Freezer Box Grid# | E3 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
