Arthrobacter phage Acai
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| Detailed Information for Phage Acai | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Andrew Adamian |
| Year Found | 2024 |
| Location Found | Glendale, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.199863 N, 118.243335 W Map |
| Discovery Notes | Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. Sample description: slightly moist and saturated; at local park (popular with dog walking), shaded area amongst trees and vegetation, isolated near wash draining from mountain next to park Depth from which sample was taken (in inches or cm): 4 cm Additional information of interest or note: One notable thing to have occurred after the sample was collected and sealed was the swelling of the bag and buildup of moisture along the sides of the plastic bag. AL Quarter and Lab section: F24-1C Instructor: Dr. Bouklas & Dr. Velez Ramirez Teaching Assistant: Ally Aves Undergraduate Assistant(s): Ellie Gorham & Jolie Vo Team #1 Name: Phagetastic Phour (P4) Team #1 Members: Amanda Streva, Carissa Liu, Audrey Qian, Isabella Torosian Team #2 Name: Phairly Odd Phages (POP) Team #2 Members: Khushi Choudhary, Andrew Adamian, Stephanie Anebere, Kaiden Burke |
| Naming Notes | Acronym of team members’ first letter of first name |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Description of plaque morphology (e.g., halos, clear or turbid): clear, circular, symmetric, pinpoint, sharp margins Average size (and size range) diameter in mm: small, 0.5-1 mm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
