| Detailed Information for Phage Acai | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Andrew Adamian |
| Year Found | 2024 |
| Location Found | Glendale, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.199863 N, 118.243335 W Map |
| Discovery Notes | Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. Sample description: slightly moist and saturated; at local park (popular with dog walking), shaded area amongst trees and vegetation, isolated near wash draining from mountain next to park Depth from which sample was taken (in inches or cm): 4 cm Additional information of interest or note: One notable thing to have occurred after the sample was collected and sealed was the swelling of the bag and buildup of moisture along the sides of the plastic bag. AL Quarter and Lab section: F24-1C Instructor: Dr. Bouklas & Dr. Velez Ramirez Teaching Assistant: Ally Aves Undergraduate Assistant(s): Ellie Gorham & Jolie Vo Team #1 Name: Phagetastic Phour (P4) Team #1 Members: Amanda Streva, Carissa Liu, Audrey Qian, Isabella Torosian Team #2 Name: Phairly Odd Phages (POP) Team #2 Members: Khushi Choudhary, Andrew Adamian, Stephanie Anebere, Kaiden Burke |
| Naming Notes | Acronym of team members’ first letter of first name |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 7145 |
| Genome length (bp) | 57878 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCT |
| GC Content | 50.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AU |
| Subcluster | AU1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewInked (None) Acai (AU1) AreFloNak (AU1) Breylor17 (AU1) Brunswick (AU1) CapnMurica (AU1) CastorTray (AU1) Darby (AU1) DevitoJr (AU1) ElephantMan (AU1) Gordon (AU1) Issa (AU1) LucySwiss (AU1) MiniBagel (AU1) Nightmare (AU1) Niktson (AU1) Nivinsha (AU1) ScienceWizSam (AU1) Synepsis (AU1) Tatanka (AU1) Teacup (AU1) Tenno (AU1) Trustiboi (AU1) Bouchard (AU2) Chlochlo (AU2) Giantsbane (AU2) LilHuddy (AU2) Phaby (AU2) Shepard (AU2) Tokki (AU2) Ingrid (AU3) Loretta (AU3) Zippen (AU3) Caterpillar (AU4) MediumFry (AU4) Makai (AU5) Truckee (AU5) Argan (AU6) BarbieDoll (AU6) Biscute (AU6) GantcherGoblin (AU6) KevinMinion (AU6) Kinny (AU6) Leathea (AU6) Lewando (AU6) Navi1117 (AU6) Phaila (AU6) Renaldo (AU6) Tenney120 (AU6) TrixiePhattel (AU6) Uzumaki (AU6) Zeina (AU6) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Description of plaque morphology (e.g., halos, clear or turbid): clear, circular, symmetric, pinpoint, sharp margins Average size (and size range) diameter in mm: small, 0.5-1 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAcai_1 Acai_2 Acai_3 Acai_4 Acai_5 Acai_6 Acai_7 Acai_8 Acai_9 Acai_10 Acai_11 Acai_12 Acai_13 Acai_14 Acai_15 Acai_16 Acai_17 Acai_18 Acai_19 Acai_20 Acai_21 Acai_22 Acai_23 Acai_24 Acai_25 Acai_26 Acai_27 Acai_28 Acai_29 Acai_30 Acai_31 Acai_32 Acai_33 Acai_34 Acai_35 Acai_36 Acai_37 Acai_38 Acai_39 Acai_40 Acai_41 Acai_42 Acai_43 Acai_44 Acai_45 Acai_46 Acai_47 Acai_48 Acai_49 Acai_50 Acai_51 Acai_52 Acai_53 Acai_54 Acai_55 Acai_56 Acai_57 Acai_58 Acai_59 Acai_60 Acai_61 Acai_62 Acai_63 Acai_64 Acai_65 Acai_66 Acai_67 Acai_68 Acai_69 Acai_70 Acai_71 Acai_72 Acai_73 Acai_74 Acai_75 Acai_76 Acai_77 Acai_78 Acai_79 Acai_80 Acai_81 Acai_82 Acai_83 Acai_84 Acai_85 Acai_86 Acai_87 Acai_88 Acai_89 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
