| Detailed Information for Phage Alone3 |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Princess Fayemi and Chethan Chandra |
| Year Found | 2022 |
| Location Found | Saint Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 13°C |
| GPS Coordinates | 38.645378 N, 90.31408 W Map |
| Discovery Notes | The soil was collected near grass, and it was taken from a depth of 2 inches.
The electron micrograph is labeled as if it contains the phage "Cursive," but this is due to a file sorting error. The micrograph contains Alone3 |
| Naming Notes | This phage was named "Alone 3" because it was the third soil sample, and we collected it far away from the group we were sampling with. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 21, 2023 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 161 |
| Genome length (bp) | 115924 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 197 bp |
| GC Content | 53.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BS |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Number of Genes | 191 |
| Number of tRNAs | 30 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR725492 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |