| Detailed Information for Phage Anandi | |
| Discovery Information | |
| Isolation Host | Arthrobacter pascens NRRL B-1814 |
| Found By | Pranjal Bhartia |
| Year Found | 2025 |
| Location Found | Atlanta, GA United States |
| Finding Institution | Emory University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 33.79809 N, 84.3118 W Map |
| Discovery Notes | Soil was collected from wet mud near an unnamed pond near Candler Lake in Lullwater Nature Preserve. The soil was damp, clumpy, and contained some roots. Two rounds of purification were used to obtain the homogenous stock. |
| Naming Notes | This phage is named after Anandi Gopal Joshi, the first female physician in India. After the death of her child due to insufficient medical care, she moved to the US to study and medicine and then she returned to practice in India. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 646 |
| Genome length (bp) | 57246 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCT |
| GC Content | 51.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AU |
| Subcluster | AU4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAcai (AU1) AreFloNak (AU1) Breylor17 (AU1) Brunswick (AU1) CapnMurica (AU1) CastorTray (AU1) Darby (AU1) DevitoJr (AU1) Dinallo (AU1) ElephantMan (AU1) Gordon (AU1) Hemma (AU1) Issa (AU1) LucySwiss (AU1) MiniBagel (AU1) Nightmare (AU1) Niktson (AU1) Nivinsha (AU1) ScienceWizSam (AU1) Synepsis (AU1) Tatanka (AU1) Teacup (AU1) Tenno (AU1) Trustiboi (AU1) Westerlin (AU1) Bouchard (AU2) Chlochlo (AU2) Giantsbane (AU2) LilHuddy (AU2) Phaby (AU2) Shepard (AU2) Tipton (AU2) Tokki (AU2) Ingrid (AU3) Loretta (AU3) Anandi (AU4) Caterpillar (AU4) MediumFry (AU4) Makai (AU5) Truckee (AU5) Altjira (AU6) Argan (AU6) BarbieDoll (AU6) Barbonzobean (AU6) Biscute (AU6) GantcherGoblin (AU6) KevinMinion (AU6) Kinny (AU6) Leathea (AU6) Lewando (AU6) Muglass (AU6) Navi1117 (AU6) Phaila (AU6) PoeMe (AU6) Renaldo (AU6) Stardrop (AU6) Tenney120 (AU6) TrixiePhattel (AU6) Uzumaki (AU6) Zeina (AU6) Inked (AU7) Zippen (AU7) |
| Annotating Institution | Emory University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | 100 μL aliquots of phage in phage buffer (10 mM Tris (pH7.5), 10 mM MgSO4, 68 mM NaCl, 1 mM CaCl2) were mixed with 100 μL of late-log A. pascens B-1814 and incubated for 15 minutes on the bench to allow phage to sorb, after which 90 uL of phage buffer and 4 mL of 0.4% PYCa top agar were added to create double-layer plates. Anandi produced plaques with a mean diameter of 1 mm (range 0.5-2 mm, n=5). The plaques are very round and clear with a faint inner halo. Plaque variation is minimal; all plaques have the same basic morphology with only minor variation in size. |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAnandi_1 Anandi_2 Anandi_3 Anandi_4 Anandi_5 Anandi_6 Anandi_7 Anandi_8 Anandi_9 Anandi_10 Anandi_11 Anandi_12 Anandi_13 Anandi_14 Anandi_15 Anandi_16 Anandi_17 Anandi_18 Anandi_19 Anandi_20 Anandi_21 Anandi_22 Anandi_23 Anandi_24 Anandi_25 Anandi_26 Anandi_27 Anandi_28 Anandi_29 Anandi_30 Anandi_31 Anandi_32 Anandi_33 Anandi_34 Anandi_35 Anandi_36 Anandi_37 Anandi_38 Anandi_39 Anandi_40 Anandi_41 Anandi_42 Anandi_43 Anandi_44 Anandi_45 Anandi_46 Anandi_47 Anandi_48 Anandi_49 Anandi_50 Anandi_51 Anandi_52 Anandi_53 Anandi_54 Anandi_55 Anandi_56 Anandi_57 Anandi_58 Anandi_59 Anandi_60 Anandi_61 Anandi_62 Anandi_63 Anandi_64 Anandi_65 Anandi_66 Anandi_67 Anandi_68 Anandi_69 Anandi_70 Anandi_71 Anandi_72 Anandi_73 Anandi_74 Anandi_75 Anandi_76 Anandi_77 Anandi_78 Anandi_79 Anandi_80 Anandi_81 Anandi_82 Anandi_83 Anandi_84 Anandi_85 Anandi_86 Anandi_87 Anandi_88 Anandi_89 Anandi_90 Anandi_91 Anandi_92 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
