Arthrobacter phage Anandi
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| Detailed Information for Phage Anandi | |
| Discovery Information | |
| Isolation Host | Arthrobacter pascens NRRL B-1814 |
| Found By | Pranjal Bhartia |
| Year Found | 2025 |
| Location Found | Atlanta, GA United States |
| Finding Institution | Emory University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 33.79809 N, 84.3118 W Map |
| Discovery Notes | Soil was collected from wet mud near an unnamed pond near Candler Lake in Lullwater Nature Preserve. The soil was damp, clumpy, and contained some roots. Two rounds of purification were used to obtain the homogenous stock. |
| Naming Notes | This phage is named after Anandi Gopal Joshi, the first female physician in India. After the death of her child due to insufficient medical care, she moved to the US to study and medicine and then she returned to practice in India. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | 100 μL aliquots of phage in phage buffer (10 mM Tris (pH7.5), 10 mM MgSO4, 68 mM NaCl, 1 mM CaCl2) were mixed with 100 μL of late-log A. pascens B-1814 and incubated for 15 minutes on the bench to allow phage to sorb, after which 90 uL of phage buffer and 4 mL of 0.4% PYCa top agar were added to create double-layer plates. Anandi produced plaques with a mean diameter of 1 mm (range 0.5-2 mm, n=5). The plaques are very round and clear with a faint inner halo. Plaque variation is minimal; all plaques have the same basic morphology with only minor variation in size. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
