| Detailed Information for Phage Anekin | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Lesley Avila |
| Year Found | 2022 |
| Location Found | Naperville, IL United States |
| Finding Institution | Benedictine University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.71679 N, 88.20451 W Map |
| Discovery Notes | I discovered Anekin from a potted plant outside the Arbor Terrace facility. The weather was hot with clear skies and was primarily sunny all day. Some of the soil that was gathered was moist due to sprinklers that were on throughout the day. However, some of the soil collected had already dried out due to the weather conditions and timing of the collection. I used a fork and dug 2 and ½ inches before collecting about ½ a sandwich Ziploc bag of soil. The soil contained some dried plant leaves, minimal rocks, and roots |
| Naming Notes | The name "Anekin" was inspired by the Star Wars series. When this project started I was really into binge-watching the series. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 342 |
| Genome length (bp) | 53469 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGTGA |
| GC Content | 62.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Oregon Coast Community College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques appear medium size and are consistently circular in form/figure. The plaques measure 2mm in diameter and have a radius of 1mm. They appear to be clear. The plaque characteristics suggest that this is a lytic phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAnekin_1 Anekin_2 Anekin_3 Anekin_4 Anekin_5 Anekin_6 Anekin_7 Anekin_8 Anekin_9 Anekin_10 Anekin_11 Anekin_12 Anekin_13 Anekin_14 Anekin_15 Anekin_16 Anekin_17 Anekin_18 Anekin_19 Anekin_20 Anekin_21 Anekin_22 Anekin_23 Anekin_24 Anekin_25 Anekin_26 Anekin_27 Anekin_28 Anekin_29 Anekin_30 Anekin_31 Anekin_32 Anekin_33 Anekin_34 Anekin_35 Anekin_36 Anekin_37 Anekin_38 Anekin_39 Anekin_40 Anekin_41 Anekin_42 Anekin_43 Anekin_44 Anekin_45 Anekin_46 Anekin_47 Anekin_48 Anekin_49 Anekin_50 Anekin_51 Anekin_52 Anekin_53 Anekin_54 Anekin_55 Anekin_56 Anekin_57 Anekin_58 Anekin_59 Anekin_60 Anekin_61 Anekin_62 Anekin_63 Anekin_64 Anekin_65 Anekin_66 Anekin_67 Anekin_68 Anekin_69 Anekin_70 Anekin_71 Anekin_72 Anekin_73 Anekin_74 Anekin_75 Anekin_76 Anekin_77 Anekin_78 Anekin_79 Anekin_80 Anekin_81 Anekin_82 Anekin_83 Anekin_84 Anekin_85 Anekin_86 Anekin_87 Anekin_88 Anekin_89 Anekin_90 Anekin_91 Anekin_92 Anekin_93 Anekin_94 Anekin_95 Anekin_96 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ362682 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
