| Detailed Information for Phage Anekin |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Lesley Avila |
| Year Found | 2022 |
| Location Found | Naperville, IL United States |
| Finding Institution | Benedictine University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.71679 N, 88.20451 W Map |
| Discovery Notes | I discovered Anekin from a potted plant outside the Arbor Terrace facility. The weather was hot with clear skies and was primarily sunny all day. Some of the soil that was gathered was moist due to sprinklers that were on throughout the day. However, some of the soil collected had already dried out due to the weather conditions and timing of the collection. I used a fork and dug 2 and ½ inches before collecting about ½ a sandwich Ziploc bag of soil. The soil contained some dried plant leaves, minimal rocks, and roots |
| Naming Notes | The name "Anekin" was inspired by the Star Wars series. When this project started I was really into binge-watching the series. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 342 |
| Genome length (bp) | 53469 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGTGA |
| GC Content | 62.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Oregon Coast Community College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques appear medium size and are consistently circular in form/figure. The plaques measure 2mm in diameter and have a radius of 1mm. They appear to be clear. The plaque characteristics suggest that this is a lytic phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ362682 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
