| Detailed Information for Phage MidnightRain |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Devansh Bansal |
| Year Found | 2022 |
| Location Found | Los Angeles, CA United States of America |
| Finding Institution | University of Southern California |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.01717 N, 118.28575 W Map |
| Discovery Notes | After my three rounds of soil I was unable to isolate my own phage, so I adopted from a classmate, Gabe Han. He collected his sample that yielded this phage from the Rose Garden near USC. The soil sample was brown, crusty, and slightly damp, and yielded phage after an enriched isolation. |
| Naming Notes | Named after the song Midnight Rain by Taylor Swift, one of my favorite songs, and the fact that phage plaque are reminiscent of raindrops. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 7, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 699 |
| Genome length (bp) | 53674 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGTGA |
| GC Content | 62.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Southern California |
| Annotation Status | Being Annotated (Expected completion by 5/1/2023) |
| Plaque Notes | The plaque of this phage are dark gray and circular, with a noticeable darker circle in the center and a more cloudy circle surrounding it. This bullseye shape could be indicative of a partly lytic and partly lysogenic life cycle. The plaque tend to be medium-sized, though vary a bit on more crowded plates. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
152 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
