| Detailed Information for Phage Bantam |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Max Wetzel and Marissa Leopold |
| Year Found | 2015 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 44.979117 N, 92.759906 W Map |
| Discovery Notes | Soil from east bank of St Croix River, near kayak launch site, next to small stream running from the river to a walking path |
| Naming Notes | We weren't sure what to name it, so I, Max, went onto www.dictionary.reference.com and looked for a synonym for something that is small, but fierce. Bantam came up as a suggestion and I stuck with that one. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1829 |
| Genome length (bp) | 92580 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCAGCACTC |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DL |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Our plaque morphology were small and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 168 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX557272 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.3*10^7 PFU/mL |
| Date of SEA Lysate Titering |
Oct 14, 2015 |
| Pitt Freezer Box# |
24 |
| Pitt Freezer Box Grid# |
B4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |