Detailed Information for Phage Bantam |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Max Wetzel and Marissa Leopold |
Year Found | 2015 |
Location Found | River Falls, WI USA |
Finding Institution | University of Wisconsin-River Falls |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 44.979117 N, 92.759906 W Map |
Discovery Notes | Soil from east bank of St Croix River, near kayak launch site, next to small stream running from the river to a walking path |
Naming Notes | We weren't sure what to name it, so I, Max, went onto www.dictionary.reference.com and looked for a synonym for something that is small, but fierce. Bantam came up as a suggestion and I stuck with that one. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 5, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1829 |
Genome length (bp) | 92580 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGCAGCACTC |
GC Content | 64.7% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | DL |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Our plaque morphology were small and clear. |
Morphotype | Siphoviridae |
Number of Genes | 168 |
Number of tRNAs | 2 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KX557272 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.3*10^7 PFU/mL |
Date of SEA Lysate Titering |
Oct 14, 2015 |
Pitt Freezer Box# |
24 |
Pitt Freezer Box Grid# |
B4 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |