| Detailed Information for Phage BarbieDoll |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Serena George |
| Year Found | 2023 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 21°C |
| GPS Coordinates | 45.427194 N, 75.692194 W Map |
| Discovery Notes | The soil sample was collected from soil beneath trees in Byward Market Square, Downtown Ottawa. |
| Naming Notes | This phage, is named after Barbie dolls to pays homage to this widely celebrated children's toy. Barbie dolls serve as a poignant symbol of the evolving narrative of women's empowerment, embodying the idea that women can aspire to be anything they envision. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 391 |
| Genome length (bp) | 55643 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCT |
| GC Content | 51.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AU |
| Subcluster | AU6 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Overdue (Expected completion by 8/31/2024) |
| Plaque Notes | This phage creates plaques that are medium sized with diffused boundaries. |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.54 x 10^9 |
| Pitt Freezer Box# |
176 |
| Pitt Freezer Box Grid# |
A11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
