| Detailed Information for Phage BlueFeather | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Kimberly Hoh |
| Year Found | 2019 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 25°C |
| GPS Coordinates | 34.056389 N, 118.445 W Map |
| Discovery Notes | Arthrobacter phage BlueFeather was isolated by direct plating at 25°C from soil that was somewhat moist and came from a sidewalk planter outside of a block of apartments. Sample collection and initial isolation by Kimberly Hoh. Characterization by Team Phages at Work (Kristina Guardino, Blake Hobbs, Kimberly Hoh and Iphen Vuong) and Team Can’t Phage Us (Aaron Bernardino, Stephanie Demo, Edward Lee). Instructor: Amanda Freise Teaching Assistant: Andrew Kapinos Undergraduate Assistants: Oscar Lim and Emily Pham |
| Naming Notes | It is a reference to an item in one of my favorite video games. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3538 |
| Genome length (bp) | 16302 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 15 bases |
| Overhang Sequence | CCACGGTTCCCGTCC |
| GC Content | 64.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FE |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology: Bullseye Morphology Average size (and size range) diameter in mm: 5 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 25 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBlueFeather_1 BlueFeather_2 BlueFeather_3 BlueFeather_4 BlueFeather_5 BlueFeather_6 BlueFeather_7 BlueFeather_8 BlueFeather_9 BlueFeather_10 BlueFeather_11 BlueFeather_12 BlueFeather_13 BlueFeather_14 BlueFeather_15 BlueFeather_16 BlueFeather_17 BlueFeather_18 BlueFeather_19 BlueFeather_20 BlueFeather_21 BlueFeather_22 BlueFeather_23 BlueFeather_24 BlueFeather_25 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT024867 |
| Refseq Number | None yet |
| Published in Paper | Demo et al 2021, PLOS ONE: BlueFeather, the singleton that wasn’t: Shared gene content analysis supports expansion of Arthrobacter phage Cluster FE |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 116 |
| Pitt Freezer Box Grid# | C5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
