| Detailed Information for Phage Boopy |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Emma D'Alonzo |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.446 N, 79.9531 W Map |
| Discovery Notes | The soil was collected while it was raining outside.
Soil was very wet and easy to scoop.
Soil is dark brown and contains some small rocks and grass.
Sample was taken right near a stone wall.
Sample was taken right near a generally busy sidewalk.
Sample was collected when it was 62 degrees Fahrenheit outside.
Sample was collected at coordinates 40.4460 N, -79.9531 W.
Sample was collected at 11:54 AM.
Sample will be held in a backpack in an air-conditioned building for about 35 minutes until lab class starts. |
| Naming Notes | This is what I call my boyfriend and he made me name my phage this. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 40 |
| Genome length (bp) | 114220 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1568 bp |
| GC Content | 53.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DS |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are very small, about 0.5mm in diameter. They are clear throughout. |
| Morphotype | Siphoviridae |
| Number of Genes | 165 |
| Number of tRNAs | 29 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK894436 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^10 |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
G9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
