| Detailed Information for Phage BruhMoment |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Stavros Ouellette |
| Year Found | 2019 |
| Location Found | Manchester, NH United States |
| Finding Institution | University of New Hampshire at Manchester |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 18°C |
| GPS Coordinates | 42.989202 N, 71.424409 W Map |
| Discovery Notes | Found in bald spot in grass. Collected on 9-3-19 at 09:25. |
| Naming Notes | That's a bruh moment right there. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 30, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 397 |
| Genome length (bp) | 67318 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 953 bp |
| End Determination Notes | There's a double-coverage region which has one clear boundary with a precise edge (the right side) while the other side (left) gradually goes back single to double coverage over ~100 bases. I've reported a best-guess approximation of the left end of this terminal repeat. |
| GC Content | 66.3% |
| Sequencing Notes | This phage was sequenced as part of a DOGEMS sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AP |
| Subcluster | AP3 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of New Hampshire at Manchester |
| Annotation Status | In GenBank |
| Plaque Notes | Pinhole sized plaque with fuzzy edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 114 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OM818331 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
116 |
| Pitt Freezer Box Grid# |
B4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
