| Detailed Information for Phage Chocolat |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Vanessa Yuan |
| Year Found | 2015 |
| Location Found | Philadelphia , PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.2311 N, 75.4119 W Map |
| Discovery Notes | With various of calcium chloride concentration up to 50mM, the plaques appear to be bigger in room temperature compare with the plates that incubated in 30 degree Celsius and 37 degree Celsius. The plates that incubated in 30 degree Celsius it showed small clear plaques with various CaCl concentrations include 0mM, 5mM, 25mM, and 50mM. However no plaques show on plates that incubated in 37 degree Celsius. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 834 |
| Genome length (bp) | 69798 |
| Character of genome ends | Circularly Permuted |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Produces small clear plaques |
| Morphotype | Myoviridae |
| Number of Genes | 111 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX670787 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
David Dunbar |
| SEA Lysate Titer |
4.28x10^-7 pfu/uL |
| Date of SEA Lysate Titering |
Jun 18, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
B11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
