| Detailed Information for Phage RosiePosie |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Rosendo Villafuerte-Vega |
| Year Found | 2015 |
| Location Found | Immaculata, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.029722 N, 75.574167 W Map |
| Discovery Notes | Soil sample acquired behind the West Campus Apartments of Immaculata University. Soil was moderately moist. |
| Naming Notes | People have a difficult time remembering my name, so they often resort to calling me "Rosie Posie." So, why not name a phage that has some relevance to my name? It makes it more personable! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 668 |
| Genome length (bp) | 70396 |
| Character of genome ends | Circularly Permuted |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | RosiePosie forms small, clear plaques. Plaque size remained consistent during the isolation process. Low titer. Optimal incubation temperature between room temperature and 30 degrees Celsius. Grew well without CaCl2, as it did with 5mM and 50mM CaCl2; however, it did not grow at 37 degrees Celsius regardless of varying CaCl2 concentrations. RosiePosie also grew well at MgSO4 concentrations of 5, 25, and 50mM concentrations when incubated at room temperature and 30 degrees Celsius. Interestingly, a clear cluster of plaques was evident for all concentrations of MgSO4 at 37 degrees Celsius for an undiluted sample of high titer lysate. In the end, plaques were clearer in the presence of magnesium. |
| Morphotype | Myoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF185723 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Cabrini College |
| SEA Lysate Titer |
6.52 x 10^8 pfu/mL |
| Date of SEA Lysate Titering |
Jul 15, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
D4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
