Arthrobacter phage RosiePosie
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Detailed Information for Phage RosiePosie
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByRosendo Villafuerte-Vega
Year Found2015
Location FoundImmaculata, PA USA
Finding InstitutionCabrini University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.029722 N, 75.574167 W Map
Discovery NotesSoil sample acquired behind the West Campus Apartments of Immaculata University. Soil was moderately moist.
Naming NotesPeople have a difficult time remembering my name, so they often resort to calling me "Rosie Posie." So, why not name a phage that has some relevance to my name? It makes it more personable!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 17, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage668
Genome length (bp)70396
Character of genome endsCircularly Permuted
GC Content61.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAR
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesRosiePosie forms small, clear plaques. Plaque size remained consistent during the isolation process. Low titer. Optimal incubation temperature between room temperature and 30 degrees Celsius. Grew well without CaCl2, as it did with 5mM and 50mM CaCl2; however, it did not grow at 37 degrees Celsius regardless of varying CaCl2 concentrations. RosiePosie also grew well at MgSO4 concentrations of 5, 25, and 50mM concentrations when incubated at room temperature and 30 degrees Celsius. Interestingly, a clear cluster of plaques was evident for all concentrations of MgSO4 at 37 degrees Celsius for an undiluted sample of high titer lysate. In the end, plaques were clearer in the presence of magnesium.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

Click to View

RosiePosie_1

RosiePosie_2

RosiePosie_3
(terminase large subunit)

RosiePosie_4
(portal protein)

RosiePosie_5
(capsid assembly protein)

RosiePosie_6

RosiePosie_7

RosiePosie_8

RosiePosie_9

RosiePosie_10
(capsid maturation protease)

RosiePosie_11
(capsid decoration protein)

RosiePosie_12
(major capsid protein)

RosiePosie_13

RosiePosie_14

RosiePosie_15
(minor tail protein)

RosiePosie_16

RosiePosie_17

RosiePosie_18
(tail sheath protein)

RosiePosie_19

RosiePosie_20

RosiePosie_21

RosiePosie_22
(tape measure protein)

RosiePosie_23
(LysM domain protein)

RosiePosie_24
(minor tail protein)

RosiePosie_25
(minor tail protein)

RosiePosie_26
(minor tail protein)

RosiePosie_27
(minor tail protein)

RosiePosie_28

RosiePosie_29

RosiePosie_30

RosiePosie_31
(lysin A)

RosiePosie_32
(holin)

RosiePosie_33

RosiePosie_34

RosiePosie_35

RosiePosie_36

RosiePosie_37
(helix-turn-helix DNA binding domain protein)

RosiePosie_38
(baseplate wedge protein)

RosiePosie_39
(baseplate J-like protein)

RosiePosie_40

RosiePosie_41

RosiePosie_42

RosiePosie_43
(DNA repair protein, ATPase-like domain)

RosiePosie_44
(helix-turn-helix DNA binding domain protein)

RosiePosie_45

RosiePosie_46
(RecE-like endonuclease)

RosiePosie_47
(recombinase)

RosiePosie_48

RosiePosie_49

RosiePosie_50

RosiePosie_51

RosiePosie_52

RosiePosie_53

RosiePosie_54

RosiePosie_55

RosiePosie_56

RosiePosie_57

RosiePosie_58

RosiePosie_59

RosiePosie_60

RosiePosie_61

RosiePosie_62

RosiePosie_63

RosiePosie_64

RosiePosie_65

RosiePosie_66

RosiePosie_67

RosiePosie_68

RosiePosie_69

RosiePosie_70

RosiePosie_71

RosiePosie_72
(endolysin domain protein)

RosiePosie_73

RosiePosie_74

RosiePosie_75

RosiePosie_76

RosiePosie_77

RosiePosie_78

RosiePosie_79

RosiePosie_80
(cytosine DNA methylase)

RosiePosie_81
(DNA methyltransferase domain protein)

RosiePosie_82
(DNA methylase)

RosiePosie_83

RosiePosie_84

RosiePosie_85

RosiePosie_86

RosiePosie_87

RosiePosie_88

RosiePosie_89
(PD-(D/E) XK-like restriction enzyme)

RosiePosie_90
(helicase)

RosiePosie_91

RosiePosie_92
(CMP N-glycosidase)

RosiePosie_93

RosiePosie_94

RosiePosie_95
(endodeoxyribonuclease)

RosiePosie_96

RosiePosie_97

RosiePosie_98

RosiePosie_99

RosiePosie_100
(HNH endonuclease)

RosiePosie_101
(ssDNA-binding protein)

RosiePosie_102

RosiePosie_103

RosiePosie_104

RosiePosie_105
(DNA polymerase III beta subunit)

RosiePosie_106

RosiePosie_107

RosiePosie_108

RosiePosie_109
(helix-turn-helix DNA binding domain protein)

RosiePosie_110

RosiePosie_111

RosiePosie_112
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF185723
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator Cabrini College
SEA Lysate Titer 6.52 x 10^8 pfu/mL
Date of SEA Lysate Titering Jul 15, 2015
Pitt Freezer Box# 2
Pitt Freezer Box Grid# F11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload